Ratnayake Jithendra, Ramesh Niranjan, Gould Maree L, Mucalo Michael R, Dias George J
Faculty of Dentistry, Department of Oral Science, University of Otago, Dunedin, New Zealand.
Department of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
J Appl Biomater Funct Mater. 2025 Jan-Dec;23:22808000251314302. doi: 10.1177/22808000251314302.
Hydroxyapatite, renowned for its biocompatibility and osteoconductive properties, plays a fundamental role in bone regeneration owing to its resemblance to natural bone mineral, thus offering considerable potential for advancing tissue engineering strategies. In this article, the innovative integration of silicon ions into biogenic (bovine-derived) hydroxyapatite (SiBHA) via a tailored sol-gel process is reported. The resultant SiBHA scaffolds exhibited an interconnected microporous structure with a total porosity of 70% and pore dimensions ranging from 120 to 650 µm. Fourier-transform infrared spectroscopy and X-ray diffraction studies validated the effective incorporation of silicon ions into the BHA lattice, with energy-dispersive X-ray and inductively-coupled plasma mass spectrometry further confirming a Ca/P molar ratio for SiBHA between 1.63 and 1.74. Moreover, SiBHA scaffolds demonstrated commendable chemical and thermal stability. Of note, SiBHA scaffolds were found to display significantly enhanced mechanical properties, including compressive strength and Young's modulus, compared to the control BHA scaffolds. In vitro assessments highlighted the capacity of SiBHA scaffolds to foster cell viability, proliferation, and osteogenic differentiation of Saos-2 cells. Immunohistochemical analysis revealed a significant increase in osteonectin expression, a key bone matrix protein, after 14 days of incubation under osteogenic conditions. These findings highlight the biocompatibility and therapeutic potential of SiBHA scaffolds, suggesting their suitability as biomaterials for dental bone regeneration applications.
羟基磷灰石因其生物相容性和骨传导特性而闻名，由于其与天然骨矿物质相似，在骨再生中发挥着重要作用，因此在推进组织工程策略方面具有巨大潜力。本文报道了通过定制的溶胶 - 凝胶工艺将硅离子创新性地整合到生物源（牛源）羟基磷灰石（SiBHA）中。所得的SiBHA支架呈现出相互连通的微孔结构，总孔隙率为70%，孔径范围为120至650μm。傅里叶变换红外光谱和X射线衍射研究验证了硅离子有效掺入BHA晶格中，能量色散X射线和电感耦合等离子体质谱进一步证实SiBHA的钙/磷摩尔比在1.63至1.74之间。此外，SiBHA支架表现出良好的化学和热稳定性。值得注意的是，与对照BHA支架相比，SiBHA支架显示出显著增强的机械性能，包括抗压强度和杨氏模量。体外评估突出了SiBHA支架促进Saos-2细胞的细胞活力、增殖和成骨分化的能力。免疫组织化学分析显示，在成骨条件下培养14天后，关键骨基质蛋白骨连接蛋白的表达显著增加。这些发现突出了SiBHA支架的生物相容性和治疗潜力，表明它们适合作为牙科骨再生应用的生物材料。
