Chen Lina, Chen Peigen, Xie Yun, Guo Jiayi, Chen Rouzhu, Guo Yingchun, Fang Cong
Center of Reproductive Medicine, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510655, China.
Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510655, China.
J Assist Reprod Genet. 2025 Feb;42(2):545-561. doi: 10.1007/s10815-024-03339-8. Epub 2025 Jan 23.
The 12-h ultradian rhythm plays a crucial role in metabolic homeostasis, but its role in ovarian aging has not been explored. This study investigates age-related changes in 12-h rhythmic gene expression across various human tissues, with a particular focus on the ovary.
We analyzed transcriptomic data from the GTEx project to examine 12-h ultradian rhythmic gene expression across multiple peripheral human tissues, exploring sex-specific patterns and age-related reprogramming of both 12-h and 24-h rhythmic gene expression.
Our findings revealed sex-dimorphic patterns in 12-h rhythmic gene expression, with females exhibiting stronger 12-h rhythms than males. Midlife (ages 40-49) was identified as a critical period for the reprogramming of both 12-h and 24-h rhythmic gene expression. The ovary was notable among other organs due to its high number of genes exhibiting 12-h rhythmic expression and a distinct pattern of rhythmic gene expression reprogramming during aging. This reprogramming involved two gene subsets: one subset adopted de novo 12-h rhythms, while another subset shifted from 24-h rhythms in younger individuals to dual 12-h and 24-h rhythms in middle-aged individuals. Both subsets were primarily associated with angiogenesis.
This study is the first to report age-related reprogramming of 12-h rhythms in human tissues, with a particular focus on the amplification of 12-h rhythms in angiogenesis-related genes in the aging ovary. These findings provide novel insights into the mechanisms structured format of the abstract text underlying ovarian aging and suggest potential therapeutic strategies targeting rhythmic gene expression in the ovary.
12小时超日节律在代谢稳态中起关键作用，但其在卵巢衰老中的作用尚未得到探索。本研究调查了不同人类组织中12小时节律性基因表达的年龄相关变化，特别关注卵巢。
我们分析了GTEx项目的转录组数据，以检查多种人类外周组织中12小时超日节律性基因表达，探索12小时和24小时节律性基因表达的性别特异性模式和年龄相关重编程。
我们的研究结果揭示了12小时节律性基因表达中的性别二态性模式，女性的12小时节律比男性更强。中年（40-49岁）被确定为12小时和24小时节律性基因表达重编程的关键时期。卵巢在其他器官中较为突出，因为其有大量基因表现出12小时节律性表达，且在衰老过程中有独特的节律性基因表达重编程模式。这种重编程涉及两个基因子集：一个子集采用全新的12小时节律，而另一个子集从年轻个体的24小时节律转变为中年个体的1 both subsets were primarily associated with angiogenesis.
本研究首次报道了人类组织中12小时节律的年龄相关重编程，特别关注衰老卵巢中血管生成相关基因的12小时节律增强。这些发现为卵巢衰老的潜在机制提供了新的见解，并提出了针对卵巢节律性基因表达的潜在治疗策略。
