Du Bingqian, Shama Aga, Zhang Yi, Chen Baolan, Bu Yongqi, Chen Pei-An, Lin Chuzhi, Liu Jie, Zheng Juan, Li Zhenjun, Chen Qingsong, Sun Yu, Fu Xi
Guangdong Provincial Engineering Research Center of Public Health Detection and Assessment, NMPA Key Laboratory for Technology Research and Evaluation of Pharmacovigilance, School of Public Health, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong, PR China.
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102200, PR China.
World Allergy Organ J. 2025 Jan 2;18(1):101017. doi: 10.1016/j.waojou.2024.101017. eCollection 2025 Jan.
Many studies reported the influence of infants' gut microbiota on atopic dermatitis (AD) postnatally, yet the role of maternal gut microbiota and plasma metabolites in infants' AD remains largely unexplored.
Sixty-three pregnant mother-infants were enrolled and followed after childbirth in Guangzhou, China. Demographic information, maternal stool and plasma samples, and records for infants' AD were collected. Maternal gut microbiota/metabolome and plasma metabolome were profiled using shotgun metagenomics and non-targeted metabolomics. Logistic regression and multi-omics analysis were used to explore characteristic maternal gut microbiota in the AD and health groups.
The α-diversity of maternal gut microbiota in health group was significantly higher than AD group (Shannon diversity = 0.02, Simpson diversity = 0.04). Short-chain fatty acids (SCFAs) producing microorganisms, including , , , and , as well as the abundance of phenylalanine, tyrosine, and tryptophan biosynthesis pathway, were enriched in health group (LDA>2 and < 0.05). Virulent factors (VFs) involved in immune modulation were enriched in the health group, while VFs involving in adhesin were enriched in the AD group ( < 0.05). Metabolomic analysis showed that a polyunsaturated fatty acid/linoleic acid, 13S-hydroxyoctadecadienoic, were negatively associated with AD in both the gut and plasma samples (FDR<0.05). Several other linoleic acids and flavonoids were negatively associated with AD (FDR<0.05). Neural network analysis revealed that microorganisms enriched in health group may produce these protective fatty acids.
Our findings show that maternal gut microorganisms/metabolites and plasma metabolites during pregnancy impact subsequent pathogenesis of infants AD. This illuminates new strategies against early AD in offspring.
许多研究报道了婴儿肠道微生物群对出生后特应性皮炎（AD）的影响，但母亲肠道微生物群和血浆代谢物在婴儿AD中的作用仍 largely unexplored。
在中国广州招募了63对母婴，并在产后进行随访。收集人口统计学信息、母亲粪便和血浆样本以及婴儿AD记录。使用鸟枪法宏基因组学和非靶向代谢组学对母亲肠道微生物群/代谢组和血浆代谢组进行分析。采用逻辑回归和多组学分析来探索AD组和健康组中母亲肠道微生物群的特征。
健康组母亲肠道微生物群的α多样性显著高于AD组（香农多样性 = 0.02，辛普森多样性 = 0.04）。健康组中富含产生短链脂肪酸（SCFAs）的微生物，包括 、 、 、 ，以及苯丙氨酸、酪氨酸和色氨酸生物合成途径的丰度（线性判别分析效应大小>2且 < 0.05）。参与免疫调节的毒力因子（VFs）在健康组中富集，而参与黏附素的VFs在AD组中富集（ < 0.05）。代谢组学分析表明，一种多不饱和脂肪酸/亚油酸、13S-羟基十八碳二烯酸在肠道和血浆样本中均与AD呈负相关（错误发现率<0.05）。其他几种亚油酸和类黄酮与AD呈负相关（错误发现率<0.05）。神经网络分析显示，健康组中富集的微生物可能产生这些具有保护作用的脂肪酸。
我们的研究结果表明，孕期母亲肠道微生物/代谢物和血浆代谢物会影响婴儿AD的后续发病机制。这为预防后代早期AD提供了新策略。
