Biront Andreas, Sillen Mart, Van Dijck Patrick, Wurm Jan
Research Group Architectural Engineering, Department of Architecture, KU Leuven, 3001 Leuven, Belgium.
Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Kasteelpark Arenberg 31, 3001 Leuven, Belgium.
Biomimetics (Basel). 2025 Jan 8;10(1):33. doi: 10.3390/biomimetics10010033.
Mycelium-based composites (MBCs) are highly valued for their ability to transform low-value organic materials into sustainable building materials, offering significant potential for decarbonizing the construction sector. The properties of MBCs are influenced by factors such as the mycelium species, substrate materials, fabrication growth parameters, and post-processing. Traditional fabrication methods involve combining grain spawn with loose substrates in a mold to achieve specific single functional properties, such as strength, acoustic absorption, or thermal insulation. However, recent advancements have focused on digital biofabrication to optimize MBC properties and expand their application scope. Despite these developments, existing research predominantly explores the use of grain spawn inoculation, with little focus on liquid spawn. Liquid spawn, however, holds significant potential, particularly in digital biofabrication, due to its ease of deposition and greater precision compared with grains. This paper, part of a digital biofabrication framework, investigates the growth kinetics of and on hemp non-woven mats, offering flexibility and mold-free fabrication using liquid inoculation. By integrating mycelium growth kinetics into digital biofabricated materials, researchers can develop more sustainable, efficient, and specialized solutions using fewer resources, enhancing the adaptability and functionality of MBCs. The experiment involved pre-cultivating and in yeast peptone dextrose (YPD) and complete yeast media (CYM) under static (ST) and shaking (SH) conditions. Four dilutions (1:10, 1:2, 1:1, and 2:1) were prepared and analyzed through imagery to assess growth kinetics. Results showed that lower dilutions promoted faster growth with full coverage, while higher dilutions offered slower growth with partial coverage. SH conditions resulted in slightly higher coverage and faster growth. To optimize the control of material properties within the digital biofabrication system, it is recommended to use CYM ST for and YPD SH for , as their growth curves show clear separation between dilutions, reflecting distinct growth efficiencies and speeds that can be selected for desired outcomes.
基于菌丝体的复合材料（MBCs）因其能够将低价值有机材料转化为可持续建筑材料而备受重视，为建筑行业脱碳提供了巨大潜力。MBCs的性能受菌丝体种类、基材、制造生长参数和后处理等因素影响。传统制造方法是将谷物菌种与松散的基材在模具中结合，以实现特定的单一功能特性，如强度、吸音或隔热。然而，最近的进展集中在数字生物制造上，以优化MBCs的性能并扩大其应用范围。尽管有这些发展，但现有研究主要探索谷物菌种接种的使用，很少关注液体菌种。然而，液体菌种具有巨大潜力，特别是在数字生物制造中，因为与谷物相比，它易于沉积且精度更高。本文作为数字生物制造框架的一部分，研究了[具体菌种1]和[具体菌种2]在大麻非织造垫上的生长动力学，通过液体接种提供了灵活性和无模具制造。通过将菌丝体生长动力学整合到数字生物制造材料中，研究人员可以使用更少的资源开发更可持续、高效和专业化的解决方案，提高MBCs的适应性和功能性。实验包括在静态（ST）和振荡（SH）条件下，在酵母蛋白胨葡萄糖（YPD）和完全酵母培养基（CYM）中预培养[具体菌种1]和[具体菌种2]。制备了四种稀释度（1:10、1:2、1:1和2:1），并通过图像分析来评估生长动力学。结果表明，较低的稀释度促进了更快的生长并实现完全覆盖，而较高的稀释度生长较慢且部分覆盖。SH条件导致覆盖率略高且生长更快。为了在数字生物制造系统中优化材料性能的控制，建议对[具体菌种1]使用CYM ST，对[具体菌种2]使用YPD SH，因为它们的生长曲线显示稀释度之间有明显的分离，反映了不同的生长效率和速度，可以根据期望的结果进行选择。
