Samarskaya Viktoriya O, Koblova Sofya, Suprunova Tatiana, Rogozhin Eugene A, Spechenkova Nadezhda, Yakunina Sofiya, Love Andrew J, Kalinina Natalia O, Taliansky Michael
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia.
Doka-Gene Technologies Ltd., Moscow Region, 141880 Rogachevo, Russia.
J Fungi (Basel). 2025 Jan 3;11(1):29. doi: 10.3390/jof11010029.
ADP-ribosylation is a reversible modification of proteins and nucleic acids, which controls major cellular processes, including DNA damage repair, cell proliferation and differentiation, metabolism, stress, and immunity in plants and animals. The involvement of ADP-ribosylation in the life cycle of and some filamentous fungi has also been demonstrated. However, the role of this process in pathogenic oomycetes has never been addressed. Here, we show that the genome contains two PARP-like protein genes (PiPARP1 and PiPARP2), and provide evidence of PARylation activity for one of them (PiPARP2). Using dsRNA-mediated RNA silencing of the PiPARP2 gene and chemical (pharmacological) inhibition of PARP activity by 3-aminobenzamide (3AB) PARP inhibitor, we demonstrate the critical functional role of ADP-ribosylation in mycelium growth. Virulence test on detached leaves also suggests an important role of ADP-ribosylation in host plant colonisation and pathogenesis. On a practical level, our data suggest that targeting the PARylation system may constitute a novel powerful approach for the management of diseases.
ADP核糖基化是蛋白质和核酸的一种可逆修饰，它控制着包括DNA损伤修复、细胞增殖与分化、代谢、应激以及动植物免疫在内的主要细胞过程。ADP核糖基化在某些病毒和丝状真菌生命周期中的作用也已得到证实。然而，这一过程在致病卵菌中的作用从未被探讨过。在此，我们表明该基因组包含两个类聚（ADP-核糖）聚合酶蛋白基因（PiPARP1和PiPARP2），并为其中一个基因（PiPARP2）提供了PAR化活性的证据。通过dsRNA介导的PiPARP2基因RNA沉默以及3-氨基苯甲酰胺（3AB）PARP抑制剂对PARP活性的化学（药理学）抑制，我们证明了ADP核糖基化在该菌菌丝体生长中的关键功能作用。对离体叶片的毒力测试也表明ADP核糖基化在该菌侵染寄主植物和致病过程中具有重要作用。在实际应用层面，我们的数据表明，针对PAR化系统可能构成一种管理该菌病害的新型有效方法。
