Wang Yan, Wu Weiwei, Wu Xiaoshu, Li Weiyu, Cui Jingjing, Long Chuannan
School of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, China.
Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, China.
J Fungi (Basel). 2025 Jan 9;11(1):49. doi: 10.3390/jof11010049.
The addition of acetic acid to cultures is usually used to inhibit the growth of heterotrophic bacteria; however, we found that acetic acid also promotes the growth of CICC41233, as well as the synthesis of pigments (MPs). Compared with no acetic acid or HCl addition, the diameter of CICC41233 colonies increased significantly under acetic acid conditions. On the sixth day of fermentation, the yield of total pigments in increased significantly by 9.97 times (compared with no acetic acid) and 13.9 times (compared with hydrochloric acid). The transcriptomics data showed that the differentially expressed genes between with acetic acid and without acetic acid were mainly involved in starch and sucrose metabolism, glycolysis/gluconeogenesis, pyruvate metabolism, TCA cycle, and oxidative phosphorylation, and that these differentially expressed genes were not involved in amino acid metabolism. Gene expression analysis showed that the relative expression levels of MP synthesis genes (, , , , , and ) were significantly up-regulated under acetic acid conditions. This study clarified the metabolic mechanism of acetic acid promoting the growth of and the synthesis of MPs, which provided some theoretical guidance for the large-scale production of MPs in the industry in future.
向培养物中添加乙酸通常用于抑制异养细菌的生长；然而，我们发现乙酸也促进了CICC41233的生长以及色素（MPs）的合成。与不添加乙酸或盐酸相比，在乙酸条件下CICC41233菌落的直径显著增加。在发酵的第六天，总色素产量显著增加，与不添加乙酸相比增加了9.97倍，与添加盐酸相比增加了13.9倍。转录组学数据表明，添加乙酸和不添加乙酸之间的差异表达基因主要参与淀粉和蔗糖代谢、糖酵解/糖异生、丙酮酸代谢、三羧酸循环和氧化磷酸化，且这些差异表达基因不参与氨基酸代谢。基因表达分析表明，MP合成基因（、、、、、和）的相对表达水平在乙酸条件下显著上调。本研究阐明了乙酸促进生长和MPs合成的代谢机制，为今后工业上大规模生产MPs提供了一些理论指导。
