Ward Selina, Lawford Harriet L S, Sartorius Benn, Lau Colleen L
UQ Centre for Clinical Research, Faculty of Health, Medicine and Behavioural Sciences, The University of Queensland, Brisbane, QLD 4006, Australia.
Trop Med Infect Dis. 2025 Jan 10;10(1):19. doi: 10.3390/tropicalmed10010019.
Integrated serological surveillance (serosurveillance) involves testing for antibodies to multiple pathogens (or species) simultaneously and can be achieved using multiplex bead assays (MBAs). This systematic review aims to describe pathogens studied using MBAs, the operational implementation of MBAs, and how the data generated were synthesised. In November and December 2023, four databases were searched for studies utilising MBAs for the integrated serosurveillance of infectious diseases. Two reviewers independently screened and extracted data regarding the study settings and population, methodology, seroprevalence results, and operational implementation elements. Overall, 4765 studies were identified; 47 were eligible for inclusion, of which 41% ( = 19) investigated multiple malaria species, and 14% performed concurrent surveillance of malaria in combination with other infectious diseases ( = 14). Additionally, 14 studies (29%) investigated a combination of multiple infectious diseases (other than malaria), and seven studies examined a combination of vaccine-preventable diseases. Haiti ( = 8) was the most studied country, followed by Ethiopia ( = 6), Bangladesh ( = 3), Kenya ( = 3), and Tanzania ( = 3). Only seven studies were found where integrated serosurveillance was the primary objective. The synthesis of data varied and included the investigation of age-specific seroprevalence ( = 25), risk factor analysis ( = 15), and spatial analysis of disease prevalence ( = 8). This review demonstrated that the use of MBAs for integrated surveillance of multiple pathogens is gaining traction; however, more research and capabilities in lower- and middle-income countries are needed to optimise and standardise sample collection, survey implementation, and the analysis and interpretation of results. Geographical and population seroprevalence data can enable targeted public health interventions, highlighting the potential and importance of integrated serological surveillance as a public health tool.
综合血清学监测（血清监测）涉及同时检测针对多种病原体（或物种）的抗体，可通过多重微珠分析（MBA）实现。本系统综述旨在描述使用MBA研究的病原体、MBA的实际操作实施，以及如何综合所生成的数据。2023年11月和12月，检索了四个数据库，以查找利用MBA进行传染病综合血清监测的研究。两名评审员独立筛选并提取了有关研究背景和人群、方法、血清阳性率结果以及实际操作实施要素的数据。总体而言，共识别出4765项研究；47项符合纳入标准，其中41%（=19）研究了多种疟原虫物种，14%同时开展了疟疾与其他传染病的监测（=14）。此外，14项研究（29%）调查了多种（非疟疾）传染病的组合，7项研究检测了疫苗可预防疾病的组合。海地（=8）是研究最多的国家，其次是埃塞俄比亚（=6）、孟加拉国（=3）、肯尼亚（=3）和坦桑尼亚（=3）。仅发现7项研究将综合血清监测作为主要目标。数据综合方式各不相同，包括特定年龄血清阳性率调查（=25）、危险因素分析（=15）以及疾病患病率的空间分析（=8）。本综述表明，使用MBA进行多种病原体的综合监测正越来越受到关注；然而，低收入和中等收入国家需要更多研究和能力，以优化和规范样本采集、调查实施以及结果的分析和解读。地理和人群血清阳性率数据可实现有针对性的公共卫生干预，凸显了综合血清学监测作为公共卫生工具的潜力和重要性。
