Lawford Harriet L S, Mayfield Helen J, Sam Filipina Amosa-Lei, Viali Satupaitea, Kamu Tito, Thomsen Robert, Lau Colleen L
Faculty of Health, Medicine, and Behavioural Sciences, University of Queensland Centre for Clinical Research (UQCCR), The University of Queensland, Brisbane, QLD, 4029, Australia.
Level 5, UQ Centre for Clinical Research, Royal Brisbane & Women's Hospital Campus, Building 71/918, Herston, QLD, 4029, Australia.
Sci Rep. 2025 Apr 12;15(1):12667. doi: 10.1038/s41598-025-96769-y.
Multiplex bead immunoassays (MBA) can detect antibody responses to multiple antigens. Using MBA data from the Surveillance and Monitoring to Eliminate Lymphatic Filariasis (LF) and Scabies from Samoa (SaMELFS) 2018, we aim to estimate national seroprevalence of neglected tropical diseases (NTDs), vaccine-preventable diseases (VPDs), and arboviruses in Samoa. A community-based serosurvey of 3851 participants aged ≥ 5 years in 35 primary sampling units (PSUs). Using MBA, dried blood spots were assayed for antibodies (Ab) from 10 pathogens: LF, trachoma, yaws, tetanus, diphtheria, rubella, measles, dengue, Zika, and chikungunya. Seroprevalence was adjusted for study design, age, and gender. NTD seroprevalence for LF was 50.8% (Bm33 Ab), 32.0% (Wb123 Ab), 20.3% (Bm14 Ab); 5.5% for trachoma; and 1.0% (Tmpa Ab) and 0.2% (Rp17 Ab) for yaws. VPD seroprevalence was 91.0% for tetanus, 83.5% for diphtheria, 79.0% for rubella, and 43.6% for measles. Arbovirus seroprevalence for dengue was 91.1% (dengue virus serotype-1 [DENV-1]), 97.2% (DENV-2), 96.9% (DENV-3), 94.7% (DENV-4); 85.7% for Zika; and 57.0% for chikungunya. Increasing age was associated with seropositivity to NTDs, arboviruses, tetanus, and measles. Clustering was highest at the household level; the strongest clustering was for DENV-3 (intraclass correlation coefficient [ICC]:0.32), Bm33 Ab (ICC:0.31), and Bm14 Ab (ICC:0.31). Integrated serosurveillance can provide a comprehensive picture of population-level immunity to multiple diseases. Our investigation into associations with seroprevalence can aid the development of evidence-based prevention, control, and elimination strategies.
多重微珠免疫分析（MBA）可检测针对多种抗原的抗体反应。利用来自2018年萨摩亚消除淋巴丝虫病（LF）和疥疮监测与监测（SaMELFS）的MBA数据，我们旨在估计萨摩亚被忽视热带病（NTDs）、疫苗可预防疾病（VPDs）和虫媒病毒的全国血清阳性率。在35个初级抽样单位（PSU）对3851名年龄≥5岁的参与者进行了基于社区的血清学调查。使用MBA，对干血斑进行检测，以检测来自10种病原体的抗体（Ab）：LF、沙眼、雅司病、破伤风、白喉、风疹、麻疹、登革热、寨卡病毒和基孔肯雅病毒。根据研究设计、年龄和性别对血清阳性率进行了调整。LF的NTD血清阳性率为50.8%（Bm33 Ab）、32.0%（Wb123 Ab）、20.3%（Bm14 Ab）；沙眼为5.5%；雅司病为1.0%（Tmpa Ab）和0.2%（Rp17 Ab）。VPD的血清阳性率为破伤风91.0%、白喉83.5%、风疹79.0%、麻疹43.6%。登革热的虫媒病毒血清阳性率为91.1%（登革热病毒1型[DENV-1]）、97.2%（DENV-2）、96.9%（DENV-3）、94.7%（DENV-4）；寨卡病毒为85.7%；基孔肯雅病毒为57.0%。年龄增加与NTDs、虫媒病毒、破伤风和麻疹的血清阳性相关。聚类在家庭层面最高；最强的聚类是DENV-3（组内相关系数[ICC]：0.32）、Bm33 Ab（ICC：0.31）和Bm14 Ab（ICC：0.31）。综合血清学监测可以全面了解人群对多种疾病的免疫力。我们对血清阳性率相关因素的调查有助于制定基于证据的预防、控制和消除策略。
