Olal Catherine, Bodmer Bianca S, Rottstegge Monika, Escudero-Pérez Beatriz, Port Julia R, Bencsik András, Nelson Emily V, Heung Michelle, Wurr Stephanie, Blake Olivia, Adam Elisa, Oestereich Lisa, Baz-Martínez Maite, Müller-Guhl Jürgen, Gallais Yann, Anjuère Fabienne, Malliere Bernard, Idoyaga Juliana, Hoenen Thomas, Muñoz-Fontela César
Bernhard Nocht Institute for Tropical Medicine, Hamburg.
German Center for Infection Research, partner site Hamburg-Borstel-Lübeck-Riems, Hamburg.
J Infect Dis. 2025 Apr 15;231(4):e615-e625. doi: 10.1093/infdis/jiae613.
Dendritic cells connect innate and adaptive immune responses. This is a particularly important immune checkpoint in the case of emerging infections against which most of the population does not have preexisting antibody immunity. In this study, we sought to test whether antibody-based delivery of Ebola virus (EBOV) antigens to dendritic cells could be used as a vaccination strategy against Ebola virus disease. Our approach was to use antibodies targeting the endocytic receptor DEC-205 present in murine and human dendritic cells, to deliver the EBOV nucleoprotein or the model antigen ovalbumin (OVA). Our findings indicate that DEC-205 targeting stimulated antigen-specific T-cell responses in mice, which resulted in protection from EBOV or recombinant EBOV-OVA challenge. An added value of this strategy was the generation of resident memory T cells. We propose that dendritic cell targeting could be used to improve T-cell responses against filoviruses, a strategy that may complement current vaccination strategies.
树突状细胞连接先天性免疫和适应性免疫反应。对于大多数人群没有预先存在抗体免疫力的新出现感染而言，这是一个特别重要的免疫检查点。在本研究中，我们试图测试基于抗体将埃博拉病毒（EBOV）抗原递送至树突状细胞是否可用作针对埃博拉病毒病的疫苗接种策略。我们的方法是使用靶向存在于小鼠和人类树突状细胞中的内吞受体DEC-205的抗体，来递送EBOV核蛋白或模型抗原卵清蛋白（OVA）。我们的研究结果表明，靶向DEC-205刺激了小鼠体内抗原特异性T细胞反应，从而使小鼠免受EBOV或重组EBOV-OVA攻击。该策略的一个附加价值是产生了驻留记忆T细胞。我们提出，靶向树突状细胞可用于改善针对丝状病毒的T细胞反应，这一策略可能补充当前的疫苗接种策略。
