AbouLaila Mahmoud, Mahmoud Maram, Wheeb Heba, Igarashi Makoto, Elkhtam Ahmed, Menshawy Soad
Department of Parasitology, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Elbehera, Egypt.
PharmD program, Egypt-Japan University of Science and Technology, New Borg El-Arab City, 21934 Alexandria, Egypt.
Vet Parasitol Reg Stud Reports. 2025 Jan;57:101170. doi: 10.1016/j.vprsr.2024.101170. Epub 2024 Nov 23.
Ascaridia galli causes weight loss, emaciation, anemia, decreased egg production, and sometimes, intestinal obstruction and death in birds, leading to economic losses in the poultry industry. This research aimed to record the occurrence of A. galli in free-range chickens at farmers' houses in three villages in Ashmoun City, Minoufiya Governorate, Egypt. Additionally, molecular characterization was conducted using internal transcribed spacer 1 (ITS-1) region PCR and sequence analysis. A total of 570 chickens from 570 small flocks, each ranging in size from 10 to 20 free-range chickens, were investigated for the presence of A. galli. DNA was extracted from 10 adult worms collected from Shoshai and Elmanil-Dowib villages and subjected to PCR amplification and sequence analysis. The overall occurrence of A. galli in Ashmoun City was 38 %, with Elmanil-Dowib village having the highest prevalence at 45 %. The oldest chickens, over one year old, had the highest occurrence rate at 56 %, while females showed a higher occurrence (44 %) than males (15 %). The highest occurrence was observed during winter (47 %). The mean intensity and abundance of A. galli in Ashmoun were 5.52 and 2.07, respectively. The ITS-1 sequences of A. galli from Ashmoun, Minoufiya, Egypt had up to 100 % identity and clustered within the same genetic clade as A. galli from Egypt, Poland, and Bangladesh. Haplotype and nucleotide diversity analyses revealed that the sequences from Ashmoun represent a single haplotype and show no nucleotide mutations. This study presents the first report of the occurrence and genetic characterization of A. galli in Baladi chickens in Ashmoun City, Minoufiya, Egypt, thereby enhancing the understanding of A. galli epidemiology in this district and developing effective control strategies.
鸡蛔虫可导致禽类体重减轻、消瘦、贫血、产蛋量下降，有时还会引起肠梗阻和死亡，给家禽业造成经济损失。本研究旨在记录埃及米努夫省阿什穆恩市三个村庄农户散养鸡中鸡蛔虫的感染情况。此外，还利用内转录间隔区1（ITS-1）区域PCR和序列分析进行了分子特征鉴定。对来自570个小群的570只散养鸡进行了鸡蛔虫感染情况调查，每个小群有10至20只散养鸡。从绍沙伊村和埃尔马尼勒-多维卜村采集的10条成年虫体中提取DNA，进行PCR扩增和序列分析。阿什穆恩市鸡蛔虫的总体感染率为38%，其中埃尔马尼勒-多维卜村的感染率最高，为45%。一岁以上的老龄鸡感染率最高，为56%，而母鸡的感染率（44%）高于公鸡（15%）。冬季的感染率最高（47%）。阿什穆恩市鸡蛔虫的平均感染强度和感染丰度分别为5.52和2.07。来自埃及米努夫省阿什穆恩市的鸡蛔虫ITS-1序列与来自埃及、波兰和孟加拉国的鸡蛔虫序列具有高达100%的同一性，并聚类在同一遗传分支内。单倍型和核苷酸多样性分析表明，阿什穆恩市的序列代表单一单倍型，未显示核苷酸突变。本研究首次报道了埃及米努夫省阿什穆恩市巴拉迪鸡中鸡蛔虫的感染情况及其遗传特征，从而增进了对该地区鸡蛔虫流行病学的了解，并有助于制定有效的防控策略。
