An Liangfeng, Fang Huihui, Zhang Ximin, Tang Jing, Gong Jiyi, Yi Yin, Tang Ming
School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China, Guizhou Normal University, Guiyang 550025, China.
Genes (Basel). 2025 Jan 20;16(1):109. doi: 10.3390/genes16010109.
Calcium-dependent protein kinases (CDPKs) are a crucial class of calcium-signal-sensing and -response proteins that significantly regulate abiotic stress. is a member of the Brassicaceae family that primarily grows in the karst regions of southwestern China, with a notable tolerance to high-calcium soils. Currently, the function of the family of genes in has yet to be explored. This study employed a comprehensive approach starting with bioinformatic methods to analyze the whole-genome sequencing data of and identified genes by combining phylogenetic characteristics and protein domain analysis. It then delved into the physicochemical properties, gene structure, chromosomal localization, phylogenetic tree, and promoter cis-acting elements of these genes. Subsequently, RNA-seq data and qRT-PCR analysis were utilized to understand the expression patterns of genes. Twenty-eight genes were found to be unevenly distributed across six chromosomes; these can be classified into four subfamilies, with many cis-acting elements in their promoter regions involved in plant growth and stress responses. Furthermore, the differential expression patterns of genes under different concentrations of calcium treatments were investigated using RNA-seq data and qRT-PCR analysis. These results are a critical first step in understanding the functions of genes, and they lay a foundation for further elucidating the adaptability and response mechanism of genes in to the karst environment.
钙依赖蛋白激酶（CDPKs）是一类关键的钙信号感知和响应蛋白，对非生物胁迫具有重要的调节作用。[植物名称]是十字花科的一员，主要生长在中国西南部的喀斯特地区，对高钙土壤具有显著的耐受性。目前，[植物名称]中该基因家族的功能尚未得到探索。本研究采用了一种综合方法，首先通过生物信息学方法分析[植物名称]的全基因组测序数据，并结合系统发育特征和蛋白质结构域分析鉴定出[X]个基因。然后深入研究了这些[X]个基因的理化性质、基因结构、染色体定位、系统发育树和启动子顺式作用元件。随后，利用RNA-seq数据和qRT-PCR分析来了解[植物名称]基因的表达模式。发现28个[植物名称]基因不均匀地分布在6条染色体上；这些基因可分为4个亚家族，其启动子区域有许多参与植物生长和胁迫响应的顺式作用元件。此外，利用RNA-seq数据和qRT-PCR分析研究了不同钙浓度处理下[植物名称]基因的差异表达模式。这些结果是了解[植物名称]基因功能的关键的第一步，为进一步阐明[植物名称]中[植物名称]基因对喀斯特环境的适应性和响应机制奠定了基础。
