Genome-Wide Identification and Characterization of the Family of Genes and Their Response to High-Calcium Stress in .

Calcium-dependent protein kinases (CDPKs) are a crucial class of calcium-signal-sensing and -response proteins that significantly regulate abiotic stress. is a member of the Brassicaceae family that primarily grows in the karst regions of southwestern China, with a notable tolerance to high-calcium soils. Currently, the function of the family of genes in has yet to be explored. This study employed a comprehensive approach starting with bioinformatic methods to analyze the whole-genome sequencing data of and identified genes by combining phylogenetic characteristics and protein domain analysis. It then delved into the physicochemical properties, gene structure, chromosomal localization, phylogenetic tree, and promoter cis-acting elements of these genes. Subsequently, RNA-seq data and qRT-PCR analysis were utilized to understand the expression patterns of genes. Twenty-eight genes were found to be unevenly distributed across six chromosomes; these can be classified into four subfamilies, with many cis-acting elements in their promoter regions involved in plant growth and stress responses. Furthermore, the differential expression patterns of genes under different concentrations of calcium treatments were investigated using RNA-seq data and qRT-PCR analysis. These results are a critical first step in understanding the functions of genes, and they lay a foundation for further elucidating the adaptability and response mechanism of genes in to the karst environment.