Lu Kun, Shan Sijie, Zeng Yanling, Yang Guliang
National Engineering Laboratory for Rice and By-Products Processing, Food Science and Engineering College, Central South University of Forestry and Technology, Changsha 410004, China.
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China.
Int J Mol Sci. 2025 Jan 10;26(2):534. doi: 10.3390/ijms26020534.
Previous research has consistently shown that high-fat diet (HFD) consumption can lead to the development of colonic inflammation. Neohesperidin (NHP), a naturally occurring flavanone glycoside in citrus fruits, has anti-inflammatory properties. However, the efficacy and mechanism of NHP in countering prolonged HFD-induced inflammation remains unclear. In this study, rats on HFD were intragastrically administered (i.g.) with NHP for 12 consecutive weeks. Results indicate that this natural compound is effective in reducing colorectal inflammation at doses of 40-80 mg/kg body weight (BW) by i.g. administration, with significant decreases in inflammation markers such as TNF-α and IL-1β levels. It also improved intestinal mucosal tissue integrity and reduced HFD-stimulated colorectal inflammation via the JAK2/STAT3 pathway. Furthermore, intestinal microbiota sequencing results show that NHP intervention significantly downregulated the Firmicutes/Bacteroidetes ratio. This ratio is closely related to the preventive role in the context of glycolipid metabolism disorder. Compared with fecal cultures of rats from the HFD group, after 48 h in vitro fermentation, those from the NHP group had distinct microbiota composition and notably higher concentrations of SCFAs. Collectively, these observations suggest that 80 mg/kg BW NHP possesses biological activities in downregulating HFD-induced colorectal inflammation by regulating intestinal flora and promoting SCFAs formation.
先前的研究一直表明，食用高脂饮食（HFD）会导致结肠炎症的发生。新橙皮苷（NHP）是柑橘类水果中天然存在的一种黄烷酮糖苷，具有抗炎特性。然而，NHP对抗长期HFD诱导的炎症的功效和机制仍不清楚。在本研究中，给食用HFD的大鼠连续12周灌胃给予NHP。结果表明，这种天然化合物通过灌胃给药，在40 - 80毫克/千克体重（BW）的剂量下可有效减轻结肠直肠炎症，炎症标志物如TNF-α和IL-1β水平显著降低。它还通过JAK2/STAT3途径改善了肠黏膜组织的完整性，并减轻了HFD刺激的结肠直肠炎症。此外，肠道微生物群测序结果表明，NHP干预显著下调了厚壁菌门/拟杆菌门的比例。该比例与糖脂代谢紊乱背景下的预防作用密切相关。与HFD组大鼠的粪便培养物相比，体外发酵48小时后，NHP组大鼠的粪便培养物具有不同的微生物群组成和明显更高的短链脂肪酸浓度。总的来说，这些观察结果表明，80毫克/千克体重的NHP通过调节肠道菌群和促进短链脂肪酸的形成，在下调HFD诱导的结肠直肠炎症方面具有生物活性。
