College of Food Science and Engineering, Dalian Ocean University, Dalian 116000, China; Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China; National R&D Branch Center for Seaweed Processing, Dalian Ocean University, Dalian 116023, China.
College of Food Science and Engineering, Dalian Ocean University, Dalian 116000, China; Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China; National R&D Branch Center for Seaweed Processing, Dalian Ocean University, Dalian 116023, China.
Int J Biol Macromol. 2024 Nov;279(Pt 3):135223. doi: 10.1016/j.ijbiomac.2024.135223. Epub 2024 Sep 4.
Laminaria digitata is a high-quality seaweed resource that is widely cultured and has good application prospects. In this study, Laminaria digitata fucoidan (LF) was extracted from Laminaria digitata, and purified using DEAE-Sepharose Fast Flow gel column to obtain four different grades. Among those, LF4 (Mw:165 kDa), mainly composed of fucose(56.80 %), had the highest total sugar (66.91 %) and sulfate (17.07 %) content. FT-RT and NMR results showed that LF4 was mainly composed of galactosylated galactofucose, and has a sulfate group attached to fucose C4. With the animal experimentation, it was revealed that hyperlipidaemic mice had significantly higher levels of TC (5.52 mmol/L), TG (2.28 mmol/L) and LDL-C (5.12 mmol/L) and significantly lower levels of HDL-C (2 mmol/L). However, LF had the efficacy in modulating the lipid metabolism disturbances induced by hyperlipidemia, as well as the ability to regulate cholesterol transport in serum. Moreover, it regulated AMPK/ACC, PPAR-α/LAXRa, Nrf2/Nqo1, TLR4/NF-κB signaling pathway genes and proteins expression in the liver. In addition, it promoted the production of beneficial short-chain fatty acids (SCFAs) while improving the composition and structure of gut microbiota, including balancing the abundance of Bacteroidota, Firmicutes, Muribaculaceae, Alloprevotella, Escherichia-Shigella, Prevotella and NK4A136. The results clearly indicated that LF4 could significantly ameliorate hyperlipidemia, suggesting its prospective application as a functional food.
海带是一种优质的海藻资源，广泛养殖，具有良好的应用前景。本研究从海带中提取了褐藻糖胶（LF），并采用 DEAE-Sepharose Fast Flow 凝胶柱对其进行纯化，得到四个不同级分。其中，LF4（Mw：165 kDa）主要由岩藻糖（56.80%）组成，总糖（66.91%）和硫酸基（17.07%）含量最高。FT-RT 和 NMR 结果表明，LF4 主要由半乳糖基化半乳糖组成，在岩藻糖 C4 上有一个硫酸基。通过动物实验发现，高脂血症小鼠的 TC（5.52 mmol/L）、TG（2.28 mmol/L）和 LDL-C（5.12 mmol/L）显著升高，HDL-C（2 mmol/L）显著降低。然而，LF 能够调节高脂血症引起的脂质代谢紊乱，调节血清胆固醇转运。此外，它调节 AMPK/ACC、PPAR-α/LAXRa、Nrf2/Nqo1、TLR4/NF-κB 信号通路基因和蛋白质在肝脏中的表达。此外，它促进有益的短链脂肪酸（SCFAs）的产生，同时改善肠道微生物群的组成和结构，包括平衡 Bacteroidota、Firmicutes、Muribaculaceae、Alloprevotella、Escherichia-Shigella、Prevotella 和 NK4A136 的丰度。结果表明，LF4 能显著改善高脂血症，有望作为功能性食品应用。
