College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128, China.
College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi 417000, China.
Oxid Med Cell Longev. 2021 Feb 1;2021:3456542. doi: 10.1155/2021/3456542. eCollection 2021.
A high-fat diet (HFD) has been previously associated with the development of diseases such as chronic colitis. While chlorogenic acid (CGA) is known to exhibit potent antioxidant, antibacterial, and anti-inflammatory properties, little is known about its effects on intestinal inflammation. In this study, we investigated the effects of CGA on intestinal inflammation in an HFD-induced obesity rat model and assessed whether these effects were related to changes in gut microbiota composition. This was achieved by examining physiological and biochemical indicators, the liver transcriptome, and the structure of the fecal microflora. CGA treatment significantly reduced HFD-induced internal organ weight gain, promoted colon tissue repair, downregulated the expression of inflammatory cytokines, and promoted the accumulation of the tight junction protein. KEGG enrichment analysis of differentially expressed genes, applied to data from the RNA-seq of rat liver tissue, revealed that CGA treatment significantly affected amino acid and lipid metabolism in the liver. Furthermore, CGA decreased the abundance of bacteria belonging to the genera , , and and increased butyric acid levels, which were positively correlated with (butyric acid producer). Moreover, the beneficial changes observed in the HFD group were not as pronounced as those in the CGA treatment group. In summary, CGA can alleviate colitis in HFD-induced obesity through its anti-inflammatory effects associated with changes in gut microbiota composition and an increase in the production of short-chain fatty acids and thus can be used as a potential drug for the treatment of this pathology.
高脂肪饮食（HFD）先前与慢性结肠炎等疾病的发展有关。虽然绿原酸（CGA）具有很强的抗氧化、抗菌和抗炎特性，但对其在肠道炎症中的作用知之甚少。在这项研究中，我们研究了 CGA 对 HFD 诱导肥胖大鼠模型中肠道炎症的影响，并评估了这些影响是否与肠道微生物群落组成的变化有关。这是通过检查生理和生化指标、肝脏转录组和粪便微生物群落的结构来实现的。CGA 处理显著减轻了 HFD 引起的内脏器官增重，促进了结肠组织修复，下调了炎症细胞因子的表达，并促进了紧密连接蛋白的积累。对大鼠肝组织 RNA-seq 数据进行的差异表达基因的 KEGG 富集分析表明，CGA 处理显著影响了肝脏中的氨基酸和脂质代谢。此外，CGA 减少了属 、 和 的细菌丰度，并增加了丁酸水平，这与 （丁酸产生菌）呈正相关。此外，CGA 治疗组的有益变化不如 CGA 处理组明显。总之，CGA 可以通过其抗炎作用减轻 HFD 诱导的肥胖中的结肠炎，这与肠道微生物群落组成的变化和短链脂肪酸的产生增加有关，因此可以用作治疗这种病理的潜在药物。
