Kenđel Adriana, Piantanida Ivo, Miljanić Snežana
Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia.
Institute Ruđer Bošković, Division of Organic Chemistry & Biochemistry, Bijenička Cesta 54, 10000 Zagreb, Croatia.
Molecules. 2025 Jan 9;30(2):231. doi: 10.3390/molecules30020231.
Aiming toward a novel, noninvasive technique, with a real-time potential application in the monitoring of the complexation of steroidal neuromuscular blocker drugs Vecuronium () and Rocuronium () with sugammadex (, medication for the reversal of neuromuscular blockade induced by or in general anesthesia), we developed proof-of-principle methodology based on surface-enhanced Raman spectroscopy (SERS). Silver nanoparticles prepared by the reduction of silver ions with hydroxylamine hydrochloride were used as SERS-active substrates, additionally aggregated with calcium nitrate as needed. The and SERS spectra were obtained within the biorelevant 5 × 10-1 × 10 M range, as well as the SERS of , though the latter was observed only in the presence of the aggregating agent. /drug complexes at a 1/1 molar ratio revealed significant spectral changes in the vibrational bands of the glucose rings and the drug steroid rings, implying that the insertion of and molecules into the cavity was not only driven by attractive electrostatic interactions between the positively charged cyclic unit of the drug and the negative carboxylate groups of cyclodextrin but also supported by hydrophobic interactions between the host cyclodextrin and the guest drug molecule. The observed changes in SERS signals are applicable in biorelevant conditions and support further studies of /drug complexes in vivo.
旨在开发一种新型非侵入性技术，该技术具有实时潜在应用价值，可用于监测甾体神经肌肉阻滞剂维库溴铵（）和罗库溴铵（）与舒更葡糖钠（，用于逆转全身麻醉中由或引起的神经肌肉阻滞的药物）的络合作用，我们基于表面增强拉曼光谱（SERS）开发了原理验证方法。通过用盐酸羟胺还原银离子制备的银纳米颗粒用作SERS活性底物，并根据需要与硝酸钙进一步聚集。在生物相关的5×10 -1×10 M范围内获得了和的SERS光谱，以及的SERS光谱，不过后者仅在存在聚集剂的情况下才能观察到。1/1摩尔比的/药物络合物在葡萄糖环和药物甾体环的振动带中显示出显著的光谱变化，这意味着和分子插入腔中不仅受到药物带正电的环状单元与环糊精的负羧基之间有吸引力的静电相互作用的驱动，还受到主体环糊精与客体药物分子之间疏水相互作用的支持。观察到的SERS信号变化适用于生物相关条件，并支持对/药物络合物在体内的进一步研究。
