Latocha Piotr, Silva Ana Margarida, Moreira Manuela M, Delerue-Matos Cristina, Rodrigues Francisca
Department of Environmental Protection and Dendrology, Institute of Horticultural Sciences, Warsaw University of Life Sciences-WULS-SGGW, 02-776 Warsaw, Poland.
REQUIMTE/LAQV, ISEP, Polytechnic of Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal.
Molecules. 2025 Jan 9;30(2):246. doi: 10.3390/molecules30020246.
Fruits are essential components of the human diet, valued for their diverse bioactive compounds with potential health-promoting properties. This study focuses on three cold-hardy species, namely , , and , examining their polyphenolic content, antioxidant/antiradical activities, scavenging capacity and effects on intestinal cell viability (Caco-2 and HT29-MTX). A comprehensive profile of their phenolic compounds was identified, in descending order of total polyphenol content: > > . Across species, 16 phenolic acids, 2 flavanols, 2 flavanones, 11 flavonols, and 3 flavones were quantified, with caffeine as a prominent compound. achieved the highest antioxidant activity, with 'Vitakola' cultivar showing almost double the antioxidant activity compared to 'Tallinn' and 'Pozdni'. By contrast, 'Geneva' and 'Pomarancheva' exhibited significantly lower activity in both FRAP and DPPH assays. Notably, cultivars showed distinct radical-scavenging capacities, particularly for superoxide, wherein 'Tallinn' and 'Pozdni' achieved the highest values. Cell viability tests on Caco-2 and HT29-MTX cells revealed a dose-dependent reduction in viability, notably stronger in Caco-2 cells. Overall, this study underscores the therapeutic potential of species.
水果是人类饮食的重要组成部分，因其含有多种具有潜在健康促进特性的生物活性化合物而受到重视。本研究聚焦于三种耐寒品种，即[品种一]、[品种二]和[品种三]，检测它们的多酚含量、抗氧化/抗自由基活性、清除能力以及对肠道细胞活力（Caco-2和HT29-MTX）的影响。确定了它们酚类化合物的全面概况，按总多酚含量降序排列为：[品种一]>[品种二]>[品种三]。在所有品种中，定量分析了16种酚酸、2种黄烷醇、2种黄烷酮、11种黄酮醇和3种黄酮，咖啡因是主要化合物。[品种一]具有最高的抗氧化活性，“Vitakola”品种的抗氧化活性几乎是“Tallinn”和“Pozdni”的两倍。相比之下，[品种二]“Geneva”和[品种三]“Pomarancheva”在FRAP和DPPH试验中均表现出显著较低的活性。值得注意的是，各品种表现出不同的自由基清除能力，尤其是对超氧化物的清除能力，其中“Tallinn”和“Pozdni”的值最高。对Caco-2和HT29-MTX细胞的细胞活力测试显示，细胞活力呈剂量依赖性降低，在Caco-2细胞中尤为明显。总体而言，本研究强调了[品种]的治疗潜力。
