Magalhães Tayane B S, Viana Amanda de O, Semedo Thiago B F, Saldanha Juliane S, Reis Nicole A Dos, Pereira Nathalia de A, Barros Rachel V P de, Miranda Hannah R, Almeida Gabriella C, Ozaki Desyrée Y S R, Caleiro Giovana S, Fenner Gustavo O, Vizu Fernanda P, Kraiser Theo, Carvalho Thais P, Thomazelli Luciano M, Dorlass Erick G, Arns Clarice W, Ferreira Helena L, Hingst-Zaher Erika, Rossi Rogério Vieira, Garbino Guilherme S T, Durigon Edison L, Araujo Jansen de, Aguiar Daniel M de
Laboratório de Virologia e Rickettsioses, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, Fernando Correa da Costa, 2367, Cuiabá 78060-900, Brazil.
Laboratório de Virologia Clínica e Molecular, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, Brazil.
Pathogens. 2025 Jan 11;14(1):58. doi: 10.3390/pathogens14010058.
Coronaviruses (CoV) infect a wide variety of hosts, causing epidemics in humans, birds, and mammals over the years. Bats (order Chiroptera) are one of the natural hosts of the Coronaviridae family. They represent 40% of the total number of mammal species in the Pantanal, a biodiversity hotspot in South America. Given the recent SARS-CoV-2 pandemic, we investigated the presence of CoV in bats captured in the Brazilian Pantanal. Oral and rectal swabs collected in 2021 from 419 bats were analyzed using Pancoronavirus-nested PCR targeting the RNA-dependent RNA-polymerase (RdRp) gene. Orthocoronavirinae was detected in 16.7% (70/419) of the bats; nine samples were sequenced, confirming that (4), (2), (1), (1), and cf. (1) collected in buildings formally used by humans were infected by genera. This is the first description of in bats from the Pantanal. As they are natural reservoirs of CoVs, constant monitoring of bats is important to comprehend the epidemiology of emerging viruses, especially in the Pantanal biome.
冠状病毒（CoV）可感染多种宿主，多年来在人类、鸟类和哺乳动物中引发疫情。蝙蝠（翼手目）是冠状病毒科的天然宿主之一。它们占南美洲生物多样性热点地区潘塔纳尔湿地哺乳动物物种总数的40%。鉴于近期新型冠状病毒肺炎大流行，我们调查了在巴西潘塔纳尔湿地捕获的蝙蝠中冠状病毒的存在情况。使用针对RNA依赖RNA聚合酶（RdRp）基因的泛冠状病毒巢式PCR对2021年从419只蝙蝠采集的口腔和直肠拭子进行分析。在16.7%（70/419）的蝙蝠中检测到正冠状病毒亚科；对9个样本进行了测序，证实从人类正式使用过的建筑物中采集的（4）、（2）、（1）、（1）和cf.（1）被属感染。这是潘塔纳尔湿地蝙蝠中首次对的描述。由于蝙蝠是冠状病毒的天然宿主，持续监测蝙蝠对于了解新兴病毒的流行病学很重要，尤其是在潘塔纳尔生物群落中。
