AlAzzam Njood F, Bajunaid Salwa O, Baras Bashayer H, Mitwalli Heba A, Weir Michael D, Xu Hockin H K
Department of Prosthetic Sciences, College of Dentistry, King Saud University, Riyadh 60169-15, Saudi Arabia.
Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 60169-15, Saudi Arabia.
Polymers (Basel). 2025 Jan 18;17(2):228. doi: 10.3390/polym17020228.
Polymethyl methacrylate (PMMA) is ideal for denture bases but is prone to biofilm accumulation, leading to denture stomatitis (DS), often involving . Dimethylaminohexadecyl methacrylate (DMAHDM) and 2-methacryloyloxyethyl phosphorylcholine (MPC) are introduced into dental materials for their antimicrobial and protein-repellent properties. This study investigates the effects of incorporating dimethylaminohexadecyl methacrylate (DMAHDM) and 2-methacryloyloxyethyl phosphorylcholine (MPC) into heat-polymerized (HP) and 3D-printed (3DP) denture base resins on microbial adhesion and cytotoxicity.
HP and 3DP denture base specimens were prepared using varying concentrations of DMAHDM and MPC. Microbial adhesion was quantified using CFU counts of , and cytotoxicity was assessed via an MTT assay using fibroblast cells after 24 h, 3 days, and 7 days.
Both DMAHDM and MPC significantly reduced the CFU counts in both HP and 3DP materials; the combination of 1.5% DMAHDM and 3% MPC exhibited the most substantial antimicrobial effects. Cytotoxicity results varied between materials and time points; however, all treated groups maintained cell viability above the 70% threshold, indicating no significant cytotoxic effects.
Incorporating DMAHDM and MPC into denture base resins can effectively reduce microbial adhesion while maintaining acceptable cytotoxicity levels.
聚甲基丙烯酸甲酯（PMMA）是义齿基托的理想材料，但容易形成生物膜堆积，导致义齿性口炎（DS），通常累及……。甲基丙烯酸十六烷基二甲氨基酯（DMAHDM）和2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）因其抗菌和抗蛋白吸附特性被引入牙科材料。本研究调查了将甲基丙烯酸十六烷基二甲氨基酯（DMAHDM）和2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）加入热聚合（HP）和3D打印（3DP）义齿基托树脂中对微生物黏附和细胞毒性的影响。
使用不同浓度的DMAHDM和MPC制备HP和3DP义齿基托标本。通过……的菌落形成单位（CFU）计数来量化微生物黏附，并在24小时、3天和7天后使用成纤维细胞通过MTT试验评估细胞毒性。
DMAHDM和MPC均显著降低了HP和3DP材料中的CFU计数；1.5% DMAHDM和3% MPC的组合表现出最显著的抗菌效果。细胞毒性结果因材料和时间点而异；然而，所有处理组的细胞活力均维持在70%阈值以上，表明无显著细胞毒性作用。
将DMAHDM和MPC加入义齿基托树脂中可有效减少微生物黏附，同时保持可接受的细胞毒性水平。
