Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, 100050, China.
Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, 100050, China; Department of Orthodontics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, 116011, China.
J Dent. 2018 Dec;79:39-45. doi: 10.1016/j.jdent.2018.09.007. Epub 2018 Sep 21.
Heat-cured resins are commonly used in orthodontics; however, there is a high incidence of caries, periodontal diseases and denture-induced stomatitis. The objectives of this study were to: (1) develop a new bioactive polymethyl methacrylate (PMMA) resin containing 2-methacryloyloxyethyl phosphorylcholine (MPC) and quaternary ammonium dimethylaminohexadecyl methacrylate (DMAHDM), and (2) investigate the effects on mechanical properties, protein-repellency and antibacterial properties.
MPC and DMAHDM were mixed into a commercial acrylic resin (Nature Cryl™ MC). Mechanical properties were measured in three-point flexure. Surface roughness was assessed using atomic force microscopy (AFM). Protein adsorption onto the PMMA resin was measured using a micro bicinchoninic acid (BCA) method. A human saliva microcosm model was used to investigate the live/dead staining and metabolic activity of the biofilms.
Incorporation of 3% MPC and 1.5% DMAHDM into PMMA resin achieved protein repellent and antibacterial capabilities, without compromising the mechanical properties. PMMA resin with 3% MPC + 1.5% DMAHDM had protein adsorption that was 1/6 that of a commercial control (p < 0.05). The PMMA resin with 3% MPC + 1.5% DMAHDM had much greater reduction in biofilm growth than using MPC or DMAHDM alone (p < 0.05).
A bioactive PMMA resin with a combination of strong protein-repellent and antibacterial capabilities was developed for the first time. The new resin greatly reduced the biofilm growth and metabolic activity, without compromising its mechanical properties.
Novel PMMA resin is promising for applications in orthodontic retainers and orthodontic appliances to reduce biofilm activity and protein adsorption around the resin.
热固性树脂在正畸中被广泛应用；然而，龋齿、牙周病和义齿性口炎的发病率很高。本研究的目的是：（1）开发一种新的含 2-（甲基丙烯酰氧）乙基磷酰胆碱（MPC）和季铵盐基二甲基氨基己基甲基丙烯酸酯（DMAHDM）的生物活性聚甲基丙烯酸甲酯（PMMA）树脂；（2）研究其对机械性能、蛋白质排斥性和抗菌性能的影响。
将 MPC 和 DMAHDM 混合到一种商业丙烯酸树脂（Nature Cryl™ MC）中。通过三点弯曲试验测量机械性能。使用原子力显微镜（AFM）评估表面粗糙度。使用微量双缩脲（BCA）法测量 PMMA 树脂对蛋白质的吸附。使用人唾液微环境模型研究生物膜的活/死染色和代谢活性。
将 3%的 MPC 和 1.5%的 DMAHDM 掺入 PMMA 树脂中，实现了蛋白质排斥和抗菌能力，同时不影响机械性能。PMMA 树脂中含有 3%的 MPC+1.5%的 DMAHDM，其蛋白质吸附量仅为商业对照品的 1/6（p<0.05）。PMMA 树脂中含有 3%的 MPC+1.5%的 DMAHDM，其生物膜生长减少幅度明显大于单独使用 MPC 或 DMAHDM（p<0.05）。
首次开发出具有强蛋白质排斥和抗菌能力的生物活性 PMMA 树脂。新型树脂大大降低了生物膜的生长和代谢活性，同时不影响其机械性能。
新型 PMMA 树脂有望应用于正畸保持器和正畸器械，以减少树脂周围的生物膜活性和蛋白质吸附。
