Song Wangdi, Zhang Taifeng, Wang Yunyun, Xue Shengnan, Zhang Yan, Zhang Genlin
State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China.
Key Laboratory of Xinjiang Endemic Phytomedicine Resources Ministry of Education, College of Pharmacy, Shihezi University, Shihezi 832003, China.
Nutrients. 2025 Jan 9;17(2):225. doi: 10.3390/nu17020225.
Polysaccharides from are known to have several bioactive effects. Previous studies have found that low-molecular-weight polysaccharide (GP1) is degraded by and promotes the production of beneficial bacteria and metabolites, which improves immune disorder and intestinal injury, and then enhances the body's immune regulation ability. However, the immune regulation effect of GP1 on a healthy body has not been studied. In this study, we aimed to reveal the immune enhancement effect and mechanism of GP1 on healthy mice.
The cytotoxicity and immunomodulatory activity of GP1 were analyzed by cell experiment; the effects of GP1 on antioxidation, immune regulation and gut microbiota structure of healthy body were studied in vivo. In addition, the mechanism of GP1 enhancing immune response of healthy body was analyzed by multi-omics.
The results show that GP1 enhanced the immune function of healthy mice by increasing the index of immune organs, improving the organizational structure of immune organs, and increasing the secretion of immune cytokines and immunoglobulin. GP1 also increased the contents of antioxidant factors such as total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in various organs and reduced the content of oxide malondialdehyde (MDA), thus enhancing the body's antioxidant capacity, promoting cell proliferation and prolonging life. Moreover, GP1 promoted the proliferation of beneficial bacteria, including , , , and , and the production of characteristic metabolites (collectively referred to as postbiotics), including α-tocopherol, arachidonic acid, melibiose, taurine, and nicotinic acid. These beneficial bacteria and postbiotics have been proven to have health maintaining functions.
GP1 promoted the proliferation of beneficial bacteria and increased the production of postbiotics, which should be the mechanism of its beneficial effect. It is expected to be a promising immune dietary supplement.
已知[具体来源]的多糖具有多种生物活性作用。先前的研究发现，低分子量[具体名称]多糖（GP1）可被[具体物质]降解，并促进有益细菌和代谢产物的产生，从而改善免疫紊乱和肠道损伤，进而增强机体的免疫调节能力。然而，GP1对健康机体的免疫调节作用尚未得到研究。在本研究中，我们旨在揭示GP1对健康小鼠的免疫增强作用及其机制。
通过细胞实验分析GP1的细胞毒性和免疫调节活性；在体内研究GP1对健康机体抗氧化、免疫调节和肠道微生物群结构的影响。此外，通过多组学分析GP1增强健康机体免疫反应的机制。
结果表明，GP1通过增加免疫器官指数、改善免疫器官组织结构以及增加免疫细胞因子和免疫球蛋白的分泌来增强健康小鼠的免疫功能。GP1还增加了各器官中总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）等抗氧化因子的含量，降低了丙二醛（MDA）的含量，从而增强了机体的抗氧化能力，促进细胞增殖并延长寿命。此外，GP1促进了有益细菌的增殖，包括[具体细菌名称1]、[具体细菌名称2]、[具体细菌名称3]和[具体细菌名称4]，以及特征性代谢产物（统称为后生元）的产生，包括α-生育酚、花生四烯酸、蜜二糖、牛磺酸和烟酸。这些有益细菌和后生元已被证明具有维持健康的功能。
GP1促进了有益细菌的增殖并增加了后生元的产生，这应该是其有益作用的机制。有望成为一种有前途的免疫膳食补充剂。
