Bussler Weston, DeZego Katelyn, Chandler Holli, Reid Robert W, Komarnytsky Slavko
Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Kannapolis, NC 28081, USA.
Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, 400 Dan Allen Drive, Raleigh, NC 27695, USA.
Nutrients. 2025 Jan 18;17(2):344. doi: 10.3390/nu17020344.
While a balanced diet can fulfill most nutritional needs, optimizing the composition of specific foods like broccoli can amplify their health benefits.
BACKGROUND/OBJECTIVES: Broccoli ( L. Italica group) is a widely consumed cruciferous vegetable valued for its gastrointestinal and immune health benefits. However, the individual contributions and interactions of broccoli glucosinolates, as they hydrolyze into bioactive isothiocyanates, remain poorly understood.
This study investigated mixtures of four major aliphatic glucosinolates-glucoraphanin, gluconapin, progoitrin, and sinigrin-in individual and combinational models to assess their effects on human colorectal cell proliferation.
Combination index analysis revealed moderate to strong antagonistic interactions among these glucosinolates, with the most significant antagonism observed during enzymatic hydrolysis by myrosinase. Mixture analysis identified an optimal glucosinolate ratio including glucoraphanin (81-84%), gluconapin (9-19%), and others (0-7%) to maximize their antiproliferative effects (adjusted R > 0.80). This optimal profile was achievable within the target broccoli mapping population. Testing the near-optimal VB067 isogenic broccoli line showed a 44% increase in antiproliferative activity compared to the initial breeding parent or an average sister line.
This study highlights the potential of leveraging nutrient-nutrient interactions to guide molecular breeding and produce functional varieties of cruciferous vegetables with optimized health benefits.
虽然均衡饮食可以满足大多数营养需求，但优化西兰花等特定食物的成分可以增强其健康益处。
背景/目的：西兰花（意大利甘蓝组）是一种广泛食用的十字花科蔬菜，因其对胃肠道和免疫健康有益而受到重视。然而，西兰花硫代葡萄糖苷水解为生物活性异硫氰酸盐后的个体贡献和相互作用仍知之甚少。
本研究在个体和组合模型中研究了四种主要脂肪族硫代葡萄糖苷——萝卜硫苷、芥子油苷、丙基硫氧嘧啶和黑芥子硫苷酸钾——的混合物，以评估它们对人结肠癌细胞增殖的影响。
组合指数分析显示这些硫代葡萄糖苷之间存在中度至强拮抗相互作用，在黑芥子酶酶解过程中观察到最显著的拮抗作用。混合物分析确定了一种最佳硫代葡萄糖苷比例，包括萝卜硫苷（81 - 84%）、芥子油苷（9 - 19%）和其他成分（0 - 7%），以最大化它们的抗增殖作用（调整后R>0.80）。在目标西兰花作图群体中可以实现这种最佳配置。测试接近最佳的VB067同基因西兰花品系，其抗增殖活性比初始育种亲本或平均姐妹系提高了44%。
本研究强调了利用营养物质 - 营养物质相互作用来指导分子育种并培育出具有优化健康益处的十字花科蔬菜功能品种的潜力。
