Aydin Nadide, Kander Thomas, Schött Ulf, Hafizi Sassan
School of Medicine, Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DT, UK.
Department of Clinical Sciences, Lund, Institution for Anaesthesiology & Intensive Care, Lund University, 22185 Lund, Sweden.
Nutrients. 2025 Jan 19;17(2):348. doi: 10.3390/nu17020348.
BACKGROUND/OBJECTIVES: Vitamin K-dependent proteins (VKDPs) all commonly possess specially modified γ-carboxyglutamic acid residues created in a vitamin K-dependent manner. Several liver-derived coagulation factors are well characterised VKDPs. However, much less is known about extrahepatic VKDPs, which are more diverse in their molecular structures and functions, and some of which have been implicated in inflammatory disorders. Vitamin K metabolism was shown to be impaired in critically ill patients, in whom systemic inflammation and sepsis are common features. Therefore, the aim of this study was to investigate the effect of vitamin K administration to these patients on their circulating levels of selected VKDPs. A particular novelty of this study was the measurement of specifically carboxylated forms of these proteins in addition to their overall levels.
Blood samples were taken from 47 patients in the intensive care unit before and approximately 24 h after intravenous vitamin K1 (10 mg) administration, and proteins were analysed by specific immunoassay.
Vitamin K1 induced increases in plasma levels of carboxylated osteocalcin and total Gas6 ( = 0.0002 and = 0.0032, respectively). No changes were detected in levels of carboxylated Gas6 or PIVKA-II (undercarboxylated prothrombin), although the latter positively correlated with undercarboxylated osteocalcin (r = 0.38).
Injected vitamin K1 increases the blood levels of two distinct VKDPs in critically ill patients, both of which have been implicated in inflammation regulation, including the increased carboxylation of one of them.
背景/目的：维生素K依赖蛋白（VKDPs）通常都具有以维生素K依赖方式产生的特殊修饰的γ-羧基谷氨酸残基。几种肝脏来源的凝血因子是特征明确的VKDPs。然而，对于肝外VKDPs的了解却少得多，它们在分子结构和功能上更加多样，其中一些与炎症性疾病有关。研究表明，危重症患者的维生素K代谢受损，全身炎症和脓毒症是这些患者的常见特征。因此，本研究的目的是调查给这些患者补充维生素K对其循环中选定VKDPs水平的影响。本研究的一个特别新颖之处在于，除了测量这些蛋白质的总体水平外，还测量了它们的特定羧化形式。
在重症监护病房的47例患者静脉注射维生素K1（10mg）之前及之后约24小时采集血样，并通过特异性免疫测定法分析蛋白质。
维生素K1使羧化骨钙素和总Gas6的血浆水平升高（分别为P = 0.0002和P = 0.0032）。羧化Gas6或异常凝血酶原（脱羧基凝血酶原）水平未检测到变化，尽管后者与脱羧基骨钙素呈正相关（r = 0.38）。
注射维生素K1可使危重症患者血液中两种不同的VKDPs水平升高，这两种蛋白均与炎症调节有关，其中一种的羧化作用增强。
