Soleimani Leila, Salehi Hassan, Pasternak Taras
Department of Horticultural Science, School of Agriculture, Shiraz University, Shiraz 7144113131, Iran.
Instituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain.
Plants (Basel). 2025 Jan 14;14(2):212. doi: 10.3390/plants14020212.
, a species native to South Africa, is characterized by its limited growth and scarcity, contributing to high production costs. Countries like China and Turkey are known for exporting globally. Tissue culture offers an efficient method for mass-producing unique and beautiful species such as This study tested Murashige and Skoog (MS) basal media supplemented with various concentrations of IBA (0.05-1.5 mg/L), NAA (0.05-0.25 mg/L), and BA (0.25-1.5 mg/L) to promote shoot proliferation. MS medium without plant growth regulators (PGRs) was also tested as a control. Different explant types (leaf, root, and inflorescence) were analyzed for their potential in direct and indirect regeneration. Inflorescence explants showed the highest callus induction with 1.5 mg/L IBA, while optimal shoot proliferation occurred at 1 mg/L IBA. Callus induction was optimal for leaf explants with 0.05 mg/L NAA and 0.25 mg/L BA, and shoot proliferation was highest at 0.05 mg/L NAA and 1 mg/L BA. Root explants achieved maximum callus induction with 0.25 mg/L BA and 0.25 mg/L NAA, with the best shoot proliferation using 0.05 mg/L NAA and 1 mg/L BA. The highest rooting percentage of regenerated shoots was obtained on ½ MS medium with 1.5 mg/L IBA.
原产于南非，其生长受限且稀缺，导致生产成本高昂。中国和土耳其等国家以向全球出口而闻名。组织培养为大量生产独特而美丽的物种提供了一种有效方法，例如本研究测试了添加不同浓度IBA（0.05 - 1.5毫克/升）、NAA（0.05 - 0.25毫克/升）和BA（0.25 - 1.5毫克/升）的Murashige和Skoog（MS）基本培养基以促进芽增殖。未添加植物生长调节剂（PGR）的MS培养基也作为对照进行了测试。分析了不同外植体类型（叶片、根和花序）在直接和间接再生方面的潜力。花序外植体在1.5毫克/升IBA时愈伤组织诱导率最高，而在1毫克/升IBA时芽增殖最佳。叶片外植体在0.05毫克/升NAA和0.25毫克/升BA时愈伤组织诱导最佳，在0.05毫克/升NAA和1毫克/升BA时芽增殖最高。根外植体在0.25毫克/升BA和0.25毫克/升NAA时愈伤组织诱导达到最大值，在0.05毫克/升NAA和1毫克/升BA时芽增殖最佳。再生芽在添加1.5毫克/升IBA的½ MS培养基上生根率最高。
