Washington Jacqueline M, Basta Holly, De Jesus Angela Bryanne, Bendele Madison G, Cresawn Steven G, Ginser Emily K
Department of Biology and Chemistry, Alliance University, New York, NY 10004, USA.
Department of Biology, Empire State University, Saratoga Springs, NY 12866, USA.
Viruses. 2025 Jan 15;17(1):113. doi: 10.3390/v17010113.
Six novel phages belonging to the family were isolated using as a host. Phages MuffinTheCat, Badulia, DesireeRose, Bee17, SCoupsA, and LuzDeMundo were purified from environmental samples by students participating in the Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program at Alliance University, New York. The phages have linear dsDNA genomes 15,438-15,636 bp with 112-120 bp inverted terminal repeats. Transmission electron microscopy (TEM) imaging analysis revealed that the six novel phages have six-sided icosahedral double-layered capsids with an internal lipid membrane that occasionally forms protruding nanotubules. Annotation analysis determined that the novel phages all have 32-34 protein-coding genes and no tRNAs. Like other , the phage genomes are arranged into two segments and include three highly conserved family genes that encode a DNA polymerase, double jelly-roll major capsid protein, and packaging ATPase. Although the novel bacteriophages have 91.6 to 97.5% nucleotide sequence similarity to each other, they are at most 58% similar to previously characterized genera. Consequently, these novel phages expand the diversity of the family, and we propose they form the sixth genus, .
使用[宿主名称未给出]作为宿主，分离出了六种属于该科的新型噬菌体。噬菌体MuffinTheCat、Badulia、DesireeRose、Bee17、SCoupsA和LuzDeMundo由纽约联盟大学参与科学教育联盟噬菌体猎人推进基因组学和进化科学（SEA-PHAGES）项目的学生从环境样本中纯化得到。这些噬菌体具有线性双链DNA基因组，长度为15,438 - 15,636 bp，带有112 - 120 bp的反向末端重复序列。透射电子显微镜（TEM）成像分析表明，这六种新型噬菌体具有六面体二十面体双层衣壳，带有内部脂质膜，并偶尔形成突出的纳米管。注释分析确定，这些新型噬菌体均具有32 - 34个蛋白质编码基因，且无tRNA。与其他[噬菌体名称未完整给出]一样，噬菌体基因组被排列成两个片段，并包括三个高度保守的家族基因，分别编码DNA聚合酶、双果冻卷主要衣壳蛋白和包装ATP酶。尽管这些新型噬菌体彼此之间的核苷酸序列相似性为91.6%至97.5%，但它们与先前已鉴定的[噬菌体属名称未完整给出]属的相似性最高为58%。因此，这些新型噬菌体扩展了[噬菌体科名称未完整给出]科的多样性，我们提议它们形成第六个属，[提议的属名称未完整给出]。
