Haotian Ruilin, Zhu Ziyu, Zhang Heao, Lv Tianjian, Tang Shanshan, Zhang Jiangjiang, Luo Aiqin, Liang Axin
Key Laboratory of Molecular Medicine and Biotherapy, the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
Ruixin Academy of Classic Learning, Beijing Institute of Technology, Beijing, 100081, China.
Biosens Bioelectron. 2025 Apr 1;273:117195. doi: 10.1016/j.bios.2025.117195. Epub 2025 Jan 21.
Covalent organic frameworks (COFs) have drawn great interest in electrochemical sensing. However, most are integrated as enrichment units or reaction carriers and are co-modified with metal nanomaterials. Few studies use the single pristine COFs as an electrochemical signal amplifier. Aza-fuzed π-conjugated COFs exhibit exceptional signal enhancement and are an effective electron transport layer for electrochemical sensing applications. In this work, different conductive aza-fuzed π-conjugated COFs were optimized by synthetic engineering. Among them, 2D crystalline COF4 with the highest conductivity (240 % via the bare electrodes) was used to modify the screen printing carbon electrode to construct a portable molecularly imprinted electrochemical biosensor for point-of-care glutathione detection. Compared with the conventional strategy of co-modifing with gold nanoparticles, the single conductive COF4 electrochemical sensor exhibited excellent detection performance and better selectivity for thiol interferents. Conductive COFs combining molecularly imprinted polymer provide a promising strategy for constructing low-cost, easy fabrication and operation, highly sensitive and selective electrochemical biosensors.
共价有机框架（COFs）在电化学传感领域引起了极大的关注。然而，大多数COFs是作为富集单元或反应载体进行整合，并与金属纳米材料进行共修饰。很少有研究将单一的原始COFs用作电化学信号放大器。氮杂稠合π共轭COFs表现出卓越的信号增强能力，并且是用于电化学传感应用的有效电子传输层。在这项工作中，通过合成工程对不同的导电氮杂稠合π共轭COFs进行了优化。其中，具有最高电导率（相对于裸电极提高了240%）的二维晶体COF4被用于修饰丝网印刷碳电极，以构建用于即时检测谷胱甘肽的便携式分子印迹电化学生物传感器。与用金纳米粒子进行共修饰的传统策略相比，单一导电COF4电化学传感器表现出优异的检测性能以及对硫醇干扰物更好的选择性。将分子印迹聚合物与导电COFs相结合为构建低成本、易于制造和操作、高灵敏度和选择性的电化学生物传感器提供了一种有前景的策略。
