Qi Yan-Ling, Zhang Hao-Tian, Li Meng, Li Wen-Jun, Hua Zheng-Shuang
Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, 230026, China.
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.
Sci Data. 2025 Jan 26;12(1):151. doi: 10.1038/s41597-025-04493-z.
Terrestrial geothermal springs, reminiscent of early Earth conditions, host diverse and abundant populations of Archaea. In this study, we reconstructed 2,949 metagenome-assembled genomes (MAGs) from 152 metagenomes collected over six years from 48 geothermal springs in Tengchong, China. Among these MAGs, 1,431 (49%) were classified as high-quality, while 1,518 (51%) were considered as medium-quality. Phylogenomic analysis revealed that these MAGs spanned 12 phyla, 27 classes, 67 orders, 147 families, 265 genera, and 475 species. Notably, 575 (19%) MAGs represented new taxa at various taxonomic levels, and 2,075 (70%) lacked nomenclature and effective descriptions. The most abundant phyla of archaeal genomes were Thermoproteota, Thermoplasmatota, and Micrarchaeota. The DRTY, ZMQ, and ZZQ geothermal springs were predominated by Archaea, particularly by Thermoproteia and Thermoplasmata. These draft genomes provide new data for studying species diversity and function within terrestrial geothermal spring archaeal communities, thus contributing to the conservation and utilization of thermophilic and hyperthermophilic microbial resources.
陆地地热泉让人联想到早期地球的环境，其中栖息着种类繁多、数量丰富的古菌。在本研究中，我们从中国腾冲48个地热泉六年内采集的152个宏基因组中重建了2949个宏基因组组装基因组（MAG）。在这些MAG中，1431个（49%）被归类为高质量，而1518个（51%）被认为是中等质量。系统基因组分析表明，这些MAG涵盖了12个门、27个纲、67个目、147个科、265个属和475个种。值得注意的是，575个（19%）MAG在不同分类水平上代表了新的分类单元，2075个（70%）缺乏命名和有效的描述。古菌基因组中最丰富 的门是热变形菌门、热原体门和微小古菌门。DRTY、ZMQ和ZZQ地热泉以古菌为主，特别是热变形菌纲和热原体纲。这些草图基因组为研究陆地地热泉古菌群落中的物种多样性和功能提供了新数据，从而有助于嗜热和超嗜热微生物资源的保护和利用。
