Hamdy Ayat, Saad Manal, ElBackly Rania, Nouh Samir, Jain Himanshu, Marei Mona
Tissue Engineering Laboratories, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Oral and Maxillofacial Surgery Specialist, Ministry of Health, Alexandria, Egypt.
Sci Rep. 2025 Jan 27;15(1):3321. doi: 10.1038/s41598-025-86408-x.
The objective of this study was to tailor an osteoinductive scaffold for alveolar bone regeneration and around immediately placed implants in extraction sockets of dogs. Tailored amorphous multiporous bioactive glass (TAMP -BG) was prepared and characterized for bioactivity and response of human alveolar bone marrow mesenchymal stem cells (hABMSCs). Extraction sockets of twenty-two male mongrel dogs received TAMP-BG in the right side around implant in the distal socket of the mandibular fourth premolar (P4), while the adjacent empty mesial socket of the same tooth was filled with the same graft. Autologous bone chips were used in the left side. Animals were euthanized at 1, 2, 4, 8 &12 weeks for histological and SEM analyses. SEM/EDX, FTIR and XRD analyses displayed formation of a bioactive hydroxycarbonated apatite layer. TAMP-BG significantly increased proliferation and migration of hABMSCs. Histologically, at the 1 week, advanced bone healing was shown in TAMP-BG group. At four weeks bone density was significantly higher forTAMP-BG (92.71% ± 1.71) versus control (62.92% ± 2.02) (P = 0.0001).At eight weeks, significant increase in width of buccal plate and height of lingual bony plate was observedfor TAMP-BG grafted implant.The socket orifice width significantly decreased for autologous bone from 1 to 12 weeks (P < 0.001), while it significantly increased for TAMP-BG (P = 0.03). We conclude that TAMP-BG can provide a preservative dynamic microenvironment following extraction up to three months which can be attributed to its unique physico-chemical characteristics.
本研究的目的是定制一种用于牙槽骨再生的骨诱导支架，并用于犬拔牙窝内即刻种植体周围。制备了定制的无定形多孔生物活性玻璃（TAMP -BG），并对其生物活性以及人牙槽骨髓间充质干细胞（hABMSCs）的反应进行了表征。22只雄性杂种犬的拔牙窝在下颌第四前磨牙（P4）远中窝的种植体周围右侧接受TAMP -BG，而同一颗牙相邻的空近中窝则填充相同的移植物。左侧使用自体骨屑。在1、2、4、8和12周时对动物实施安乐死以进行组织学和扫描电镜分析。扫描电镜/能谱分析、傅里叶变换红外光谱分析和X射线衍射分析显示形成了生物活性羟基碳酸磷灰石层。TAMP -BG显著增加了hABMSCs的增殖和迁移。组织学上，在1周时，TAMP -BG组显示出更高级的骨愈合。在4周时，TAMP -BG组的骨密度（92.71%±1.71）显著高于对照组（62.92%±2.02）（P = 0.0001）。在8周时，观察到TAMP -BG移植种植体的颊板宽度和舌侧骨板高度显著增加。自体骨的牙槽窝口宽度从1周到12周显著减小（P < 0.001），而TAMP -BG组则显著增加（P = 0.03）。我们得出结论，TAMP -BG在拔牙后长达三个月的时间内可以提供一个具有保护作用的动态微环境，这可归因于其独特的物理化学特性。
