Lee Eun Ji, Yoon Kyung Young
Department of Food and Nutrition, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Molecules. 2025 Apr 6;30(7):1635. doi: 10.3390/molecules30071635.
This study was conducted to identify the optimal conditions and evaluate the feasibility of deep eutectic solvent (DES)-based ultrasound-assisted extraction (UAE) for utilizing maca () leaves, an agricultural by-product, as functional materials. The extraction parameters influencing the recovery of saponins and polyphenols, which are major bioactive compounds, were analyzed using the Taguchi method. Results: Signal-to-noise ratios and analysis of variance indicated that the liquid-solid ratio was the most critical factor for optimizing the extraction process. The optimal extraction conditions were determined to be a liquid-solid ratio of 40 mL/g, a water content in DES of 30%, an extraction time of 30 min, and an ultrasonic power of 300 W in the DES system consisting of choline chloride and glycerin in the molar ratio of 1:2. Maca leaf extract obtained under optimized DES-based UAE conditions exhibited higher bioactive compounds content and antioxidant activity compared with that obtained by hot water extraction. Therefore, the DES-based UAE method is a promising, eco-friendly alternative for extracting bioactive compounds from maca leaves.
本研究旨在确定最佳条件，并评估基于低共熔溶剂（DES）的超声辅助提取（UAE）利用玛咖（）叶（一种农业副产品）作为功能材料的可行性。使用田口方法分析了影响主要生物活性化合物皂苷和多酚回收率的提取参数。结果：信噪比和方差分析表明，液固比是优化提取过程的最关键因素。在由摩尔比为1:2的氯化胆碱和甘油组成的DES体系中，最佳提取条件确定为液固比40 mL/g、DES含水量30%、提取时间30分钟和超声功率300 W。与热水提取法相比，在基于DES的优化UAE条件下获得的玛咖叶提取物表现出更高的生物活性化合物含量和抗氧化活性。因此，基于DES的UAE方法是从玛咖叶中提取生物活性化合物的一种有前景的、环保的替代方法。
