Ota D M, Drewinko B
Cancer Res. 1985 May;45(5):2128-31.
In this study, we investigated the influence of some of the variables of the thymidine labeling index (TLI) in human colorectal carcinoma. These variables were: cell suspensions versus tissue fragments; incubation with 5-fluoro-2'-deoxyuridine; method of tissue procurement; location in the large bowel; and TLI distribution in different areas of the tumor. Mean TLI values for cell suspensions and tissue fragments were 3.4 (range, 0.1 to 7) and 1 (0.1 to 2.6), respectively. Incubation with 5-fluoro-2'-deoxyuridine significantly reduced tritiated thymidine incorporation. There were no differences in TLI values between biopsy and surgical samples and in different areas of the large intestine. Median TLI of cell suspensions in 47 tumors was 2.25 (0.1 to 10.1). These results show that the TLI of colorectal carcinoma is low and correlates with its slow growth. Cell suspension provides a more representative and unbiased sample than tissue fragments in cell kinetics studies.
在本研究中，我们调查了人结直肠癌中胸苷标记指数（TLI）的一些变量的影响。这些变量包括：细胞悬液与组织碎片；与5-氟-2'-脱氧尿苷孵育；组织获取方法；在大肠中的位置；以及肿瘤不同区域的TLI分布。细胞悬液和组织碎片的平均TLI值分别为3.4（范围0.1至7）和1（0.1至2.6）。与5-氟-2'-脱氧尿苷孵育显著降低了氚标记胸苷的掺入。活检样本和手术样本之间以及大肠不同区域的TLI值没有差异。47个肿瘤中细胞悬液的TLI中位数为2.25（0.1至10.1）。这些结果表明，结直肠癌的TLI较低，与其生长缓慢相关。在细胞动力学研究中，细胞悬液比组织碎片提供了更具代表性和无偏性的样本。
