Adams J D, LaVoie E J, Hoffmann D
Carcinogenesis. 1985 Apr;6(4):509-11. doi: 10.1093/carcin/6.4.509.
Methods were developed to determine the biological half-lives and rates of distribution and elimination of N'-nitrosonornicotine (NNN), N'-nitrosoanatabine (NAT), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) in the F344 rat. The formation and persistence of an in vivo equilibrium between NNK and NNAL were also studied. The method consists of extraction and elution of the nitrosamines through a Clin-Elut column with ethyl acetate, followed by concentration and analysis by a gas chromatography-thermal energy analyzer. The biological half-lives for NNN and NAT were 184 and 540 min, those for NNK and NNAL ranged from 25 to 37 and 184 to 298 min, respectively. A relatively short biological half-life for the TSNA suggests a correlation with carcinogenic potency.
已开发出方法来测定F344大鼠体内N'-亚硝基去甲烟碱（NNN）、N'-亚硝基新烟草碱（NAT）、4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁酮（NNK）和4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁醇（NNAL）的生物半衰期以及分布和消除速率。还研究了NNK和NNAL之间体内平衡的形成和持续性。该方法包括通过Clin-Elut柱用乙酸乙酯萃取和洗脱亚硝胺，然后进行浓缩并通过气相色谱-热能分析仪进行分析。NNN和NAT的生物半衰期分别为184分钟和540分钟，NNK和NNAL的生物半衰期分别为25至37分钟和184至298分钟。烟草特有亚硝胺相对较短的生物半衰期表明其与致癌潜力相关。
