Ingebretsen O C, Bakken A M, Farstad M
Clin Chem. 1985 May;31(5):695-8.
In this relatively rapid liquid-chromatographic procedure the endogenous serotonin in blood platelets is quantified by fluorometry. We used this experimental procedure to estimate the platelet-release reaction as an indicator of the functional integrity of stored platelets. a decrease in the platelet-release reaction more sensitively indicates platelet changes during storage than do alterations in total ATP concentration or adenylate energy charge. The described method for quantifying serotonin is convenient enough for routine use in blood banks.
在这个相对快速的液相色谱程序中，通过荧光法对血小板中的内源性血清素进行定量。我们使用这个实验程序来评估血小板释放反应，以此作为储存血小板功能完整性的指标。与总ATP浓度或腺苷酸能量电荷的变化相比，血小板释放反应的降低更能灵敏地表明储存期间血小板的变化。所描述的血清素定量方法足够简便，可用于血库的常规检测。
