Chauhan Jayhind Kumar, Kumar Pradeep, Dubey Pawan K, Tripathi Anima
Department of Zoology, MMV, Banaras Hindu University, Varanasi, 221005, UP, India.
Centre for Genetic Disorders, Institute of Science, Banaras Hindu University, Varanasi, 221005, UP, India.
Cell Biochem Biophys. 2025 Jan 28. doi: 10.1007/s12013-025-01676-8.
Putranjiva roxburghii is an important medicinal plant utilized for remedy of female reproductive ailments. Its seed extract is being used as a uterine health booster due to the presence of several pharmaceutically important phytochemicals. However, the presence of phytochemicals in its leaf is still unexplored. The present study was designed to explore phytochemical finger printing and assessment of anti-oxidant and anti-inflammatory activities of hydroalcoholic leaf extract of P. roxburghii (HALEPR). The qualitative, quantitative phytochemical of flavonoid, phenol and HRA-MS analysis of HALEPR carried out along with antioxidant and in vitro membrane stabilization and protein denaturation assay of anti-inflammatory activity were have been analyzed. Results of qualitative phytochemical screening of HALEPR denotes the existence of phenol, flavonoids, alkaloids, coumarins, steroids, saponins, tannins, anthroquinone and carbohydrates. The quantitative phytochemical of flavonoid and phenol was done which revealed the presents of total phenol and flavonoid. High resolution accurate-mass spectrometry (HRA-MS) study was also done for the identification of bioactive compounds from the HALEPR, which showed the presence of various phytochemicals such as luteolin 3'- (3″-acetylglucuronide), luteolin 4'-methyl ether 7-glucoside, quercetin-3β-D-glucoside, 8-hydroxyluteolin 4'-methyl ether 8-glucuronide, quercetin 3-xylosyl- (1- > 2) -rha mnosyl- (1- > 6) -glucoside, quercetin-3β-D-glucoside, myricetin 3- (3-6-diacetylglucosyl) - (1- > 4) - (2″,3″-diacetylrhamnoside), apigetrin, isorhamnetin, catechin 7,3'-Di-O-β-D glucopyranoside, luteolin 7-methylglucuronide, apigenin-8-C-α -l-arabinopyranoside, naringenin 7- O-β-D-glucoside 6″-acetate,ohobanin, shogaol, ginkgetin and amoritin. The HELPER is shown to have the presence of anti-oxidant and anti-inflammatory activities as demonstrated by DPPH (1, 1-diphenyl,2-picrylhydrazyl) and membrane lysis assays. Our findings reveal the presence of phytochemicals in HALEPR that have significant antioxidant and anti-inflammatory activity. The bioactivities were identified using chemical characterization like HRA/MS and biological assessments like anti-inflammatory and antioxidant assays. Future research may focus on isolating specific molecules, conducting in vivo tests, and creating HALEPR-based formulations for clinical application as anti-inflammatory drugs.
倒捻子是一种重要的药用植物，用于治疗女性生殖疾病。由于其含有多种具有重要药用价值的植物化学物质，其种子提取物被用作子宫健康增强剂。然而，其叶片中植物化学物质的存在仍未被探索。本研究旨在探索倒捻子水醇叶提取物（HALEPR）的植物化学指纹图谱，并评估其抗氧化和抗炎活性。对HALEPR进行了黄酮类、酚类的定性、定量植物化学分析以及HRA-MS分析，并对其抗氧化、体外膜稳定和蛋白质变性抗炎活性测定进行了分析。HALEPR的植物化学定性筛选结果表明存在酚类、黄酮类、生物碱、香豆素、甾体、皂苷、单宁、蒽醌和碳水化合物。对黄酮类和酚类进行了定量植物化学分析，结果显示存在总酚和黄酮类。还进行了高分辨率精确质谱（HRA-MS）研究，以鉴定HALEPR中的生物活性化合物，结果表明存在多种植物化学物质，如木犀草素3'-（3″-乙酰葡萄糖醛酸苷）、木犀草素4'-甲基醚7-葡萄糖苷、槲皮素-3β-D-葡萄糖苷、8-羟基木犀草素4'-甲基醚8-葡萄糖醛酸苷、槲皮素3-木糖基-（1->2）-鼠李糖基-（1->6）-葡萄糖苷、槲皮素-3β-D-葡萄糖苷、杨梅素3-（3-6-二乙酰葡萄糖基）-（1->4）-（2″，3″-二乙酰鼠李糖苷）、芹菜素、异鼠李素、儿茶素7,3'-二-O-β-D-葡萄糖苷、木犀草素7-甲基葡萄糖醛酸苷、芹菜素-8-C-α-L-阿拉伯吡喃糖苷、柚皮素7-O-β-D-葡萄糖苷6″-乙酸酯、奥霍巴宁、姜辣素、银杏黄素和阿莫里辛。通过DPPH（1,1-二苯基，2-苦基肼基）和膜裂解试验表明，HALEPR具有抗氧化和抗炎活性。我们的研究结果揭示了HALEPR中存在具有显著抗氧化和抗炎活性的植物化学物质。这些生物活性是通过HRA/MS等化学表征和抗炎及抗氧化试验等生物学评估来确定的。未来的研究可能集中在分离特定分子、进行体内试验以及开发基于HALEPR的制剂作为抗炎药物用于临床应用。
