Elizondo-Luevano Joel H, Quintanilla-Licea Ramiro, Castillo-Hernández Sandra L, Sánchez-García Eduardo, Bautista-Villarreal Minerva, González-Meza Georgia M, Gloria-Garza Marcela A, Rodríguez-Luis Osvelia E, Kluz Maciej Ireneusz, Kačániová Miroslava
Faculty of Biological Sciences, Universidad Autónoma de Nuevo León, Cd., San Nicolás de los Garza 66455, Nuevo León, Mexico.
Faculty of Pharmacy, University of Salamanca, 37008 Salamanca, Spain.
Life (Basel). 2024 Sep 18;14(9):1176. doi: 10.3390/life14091176.
Plant extracts of fifteen plants of ethnomedicinal use in Mexico were analyzed to provide scientific knowledge of their medicinal properties through the evaluation of different biological activities such as anti-hemolytic, antioxidant, and cytotoxic effects in normal cells. Therefore, methanolic extracts were obtained from each of the plants by the Soxhlet extraction. The hemolytic activity in human erythrocytes was evaluated, as was their potential to protect the erythrocyte membrane against the 2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH) and 1,1-diphenyl-2-picryl hydrazyl (DPPH) radicals. Finally, the toxicity of the extracts in normal cell cultures of African green monkey kidney cells (Vero) and peripheral blood mononuclear cells (PBMC) was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction method. Most of the extracts showed low hemolytic activity and high anti-hemolytic activity as well as high selectivity indices (SI) and antioxidant effects. Extracts of , , and induced cell proliferation of the Vero cells. , , , , , and extracts showed PBMC cell proliferation. In the present study, it was observed that the evaluated extracts did not present hemolytic activity, and some presented low toxicity when Vero and PBMC cell cultures were exposed. In conclusion, traditionally used plants possess beneficial health properties, and it is hoped that this study will serve as a basis for understanding the biological effects of traditionally used plants and may complement future studies.
对墨西哥15种用于民族医学的植物提取物进行了分析，通过评估不同的生物活性，如对正常细胞的抗溶血、抗氧化和细胞毒性作用，来提供其药用特性的科学知识。因此，采用索氏提取法从每种植物中获得甲醇提取物。评估了其对人红细胞的溶血活性，以及它们保护红细胞膜免受2,2'-偶氮二（2-甲基丙脒）二盐酸盐（AAPH）和1,1-二苯基-2-苦基肼基（DPPH）自由基损伤的潜力。最后，通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）还原法测定提取物对非洲绿猴肾细胞（Vero）和外周血单个核细胞（PBMC）正常细胞培养物的毒性。大多数提取物显示出低溶血活性和高抗溶血活性以及高选择性指数（SI）和抗氧化作用。、和的提取物诱导Vero细胞增殖。、、、、和的提取物显示出PBMC细胞增殖。在本研究中，观察到所评估的提取物没有溶血活性，并且当Vero和PBMC细胞培养物暴露时，一些提取物表现出低毒性。总之，传统使用的植物具有有益的健康特性，希望本研究将作为理解传统使用植物的生物学效应的基础，并可能补充未来的研究。
