Manivasagam Vignesh K, Sankar Magesh, Garcia Caterina Bartomeu, Vishnu Jithin, Chatterjee Kaushik, Suwas Satyam, Manivasagam Geetha, Webster Thomas J
Department of Chemical Engineering, Northeastern University, Boston, MA USA.
Present Address: Department of Mechanical Engineering, Colorado State University, Fort Collins, CO USA.
In Vitro Model. 2022 May 2;1(3):273-288. doi: 10.1007/s44164-022-00016-x. eCollection 2022 Jun.
WE43 magnesium alloy was modified using surface mechanical attrition treatment (SMAT) and characterized to evaluate the influence of sub-micron surface modification on degradation rate and in vitro behavior. Modified surface was characterized for wettability, hardness, roughness, degradation rate, in vitro biocompatibility, and antibacterial activity as per the ASTM standards. The treated substrates proved to have a significant decrease in the degradation profile by creating micro pockets of oxidation channels and reducing the total delamination in comparison to the conventional heterogeneous oxide layer formed on the untreated substrate surface. Biocompatibility studies showed that this modification did not induce any toxicity to human fetal osteoblast (hFOB) cells as demonstrated by cell proliferation and enhanced calcium deposition. In fact, results showed that between the 7 day and 14 day of culture, there was an eight time increase in calcium deposition for the surface-treated magnesium alloy. Bacterial adhesion and toxicity studies were carried out using and methicillin-resistant . Bacterial toxicity studies showed that both treated and control samples were toxic to the bacteria with more dead cells. Hence, this treatment has developed a highly potential orthopedic surface with decreased biodegradability rate of WE43 and simultaneously enhanced antibacterial properties with good osteoblast cell growth and calcium deposition for faster in vitro bone growth.
采用表面机械研磨处理（SMAT）对WE43镁合金进行改性，并对其进行表征，以评估亚微米表面改性对降解速率和体外行为的影响。按照ASTM标准对改性表面的润湿性、硬度、粗糙度、降解速率、体外生物相容性和抗菌活性进行了表征。与在未处理的基体表面形成的传统非均匀氧化层相比，经处理的基体通过形成氧化通道的微坑并减少总脱层，降解曲线显著降低。生物相容性研究表明，这种改性对人胎儿成骨细胞（hFOB）没有诱导任何毒性，这通过细胞增殖和增强的钙沉积得到了证明。事实上，结果表明，在培养的第7天到第14天之间，表面处理的镁合金的钙沉积增加了8倍。使用[具体细菌名称]和耐甲氧西林[具体细菌名称]进行了细菌粘附和毒性研究。细菌毒性研究表明，处理过的样品和对照样品对细菌都有毒性，死细胞更多。因此，这种处理方法开发出了一种极具潜力的骨科表面，降低了WE43的生物降解率，同时增强了抗菌性能，具有良好的成骨细胞生长和钙沉积，可加快体外骨生长。
