Lang Huiyuan, Guo Zengwang, Wu Yu, Li Li, Liu Hongmei, Jiang Lianzhou, Wang Songxue, Ye Jin
College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
Heilongjiang Grain and Oil Food Safety Testing Industry Technology Research Institute, Harbin, Heilongjiang 150030, China.
Food Chem X. 2025 Jan 7;25:102167. doi: 10.1016/j.fochx.2025.102167. eCollection 2025 Jan.
The contamination of Alternaria toxins poses a potential risk to human health. This study developed a rapid, efficient, and environmentally friendly method for the simultaneous determination of five types of Alternaria toxins in wheat using high-precision and stable isotope liquid chromatography tandem mass spectrometry. The comparison between dilution method and solid-phase extraction method shows that the former achieves satisfactory results with a simple and convenient sample purification method. The quantitative limit range is 0.88 to 1.68 μg/kg. The recoveries are between 81.40% and 102.68%, with RSD less than 11.95%. The method was used to analyze 60 samples from the main wheat producing areas in China. The results showed that Tenuzonic acid had the highest detection rate (100%), followed by Tentoxin (95%), Alternariol (66.67%), and Alternariol monomethyl ether (53.33%). There is a certain pollution risk that needs to be taken seriously and monitoring should be strengthened.
链格孢菌毒素的污染对人类健康构成潜在风险。本研究建立了一种快速、高效且环保的方法，利用高精度稳定同位素液相色谱串联质谱法同时测定小麦中五种链格孢菌毒素。稀释法与固相萃取法的比较表明，前者通过简单便捷的样品净化方法取得了满意的结果。定量限范围为0.88至1.68μg/kg。回收率在81.40%至102.68%之间，相对标准偏差小于11.95%。该方法用于分析中国主要小麦产区的60个样品。结果表明，细交链孢菌酮酸的检出率最高（100%），其次是细交链孢菌毒素（95%）、交链孢酚（66.67%）和交链孢酚单甲醚（53.33%）。存在一定的污染风险，需要认真对待并加强监测。
