Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University, Gwangju 61186, South Korea.
Department of Chemistry, Chonnam National University, Gwangju 61186, South Korea.
Int J Food Microbiol. 2018 Mar 2;268:44-52. doi: 10.1016/j.ijfoodmicro.2018.01.003. Epub 2018 Jan 3.
Twenty-four Alternaria strains were isolated from barley grain samples. These strains were screened for the production of mycotoxins on rice medium using thin layer chromatography. All 24 strains produced at least one of the five mycotoxins (ALT, AOH, ATX-I, AME, and TeA). Three representative strains, namely EML-BLDF1-4, EML-BLDF1-14, and EML-BLDF1-18, were further analyzed using a new LC-MS/MS-based mycotoxin quantification method. This method was used to detect and quantify Alternaria mycotoxins. We used positive ion electrospray mass spectrometry with multiple reaction mode (MRM) for the simultaneous quantification of various Alternaria mycotoxins produced by these strains. Five Alternaria toxins (ALT, ATX-I, AOH, AME, and TeA) were detected and quantified. Sample preparation included methanol extraction, concentration, and injection into LC-MS/MS. Limit of detection ranged from 0.13 to 4μg/mL and limit of quantification ranged from 0.25 to 8μg/mL.
从大麦籽粒样品中分离出 24 株链格孢菌。这些菌株在大米培养基上用薄层色谱法筛选产生的霉菌毒素。24 株菌均至少产生了五种霉菌毒素中的一种（ALT、AOH、ATX-I、AME 和 TeA）。然后，使用一种新的基于 LC-MS/MS 的霉菌毒素定量方法进一步分析了三个代表性菌株，即 EML-BLDF1-4、EML-BLDF1-14 和 EML-BLDF1-18。该方法用于检测和定量这些菌株产生的各种链格孢霉菌毒素。我们使用正离子电喷雾质谱法（MRM）对这些菌株产生的各种链格孢霉菌毒素进行同时定量分析。检测和定量了五种链格孢毒素（ALT、ATX-I、AOH、AME 和 TeA）。样品制备包括甲醇提取、浓缩和注入 LC-MS/MS。检测限范围为 0.13 至 4μg/mL，定量限范围为 0.25 至 8μg/mL。
