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Functional integration of Cas9 gene into the genome of rhesus monkey: possibility of a new biomedical model?

作者信息

Wang Yi, Wu Junmo, Wang Xuan, Kang Yu, Chu Chu, Zeng Yuqiang, Chen Zhenzhen, Zhao Ziyi, Chen Xinglong, Tian Baohong, Niu Yuyu

机构信息

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming 650500, China.

Yunnan Key Laboratory of Primate Biomedical Research, Kunming 650500, China.

出版信息

Life Med. 2023 Jan 18;2(1):lnad002. doi: 10.1093/lifemedi/lnad002. eCollection 2023 Feb.

DOI:10.1093/lifemedi/lnad002
PMID:39872955
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11749692/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa11/11749692/af4a1abf84cc/lnad002_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa11/11749692/cdf089cdc67b/lnad002_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa11/11749692/af4a1abf84cc/lnad002_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa11/11749692/cdf089cdc67b/lnad002_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fa11/11749692/af4a1abf84cc/lnad002_fig2.jpg

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Functional integration of Cas9 gene into the genome of rhesus monkey: possibility of a new biomedical model?Cas9基因在恒河猴基因组中的功能整合:一种新的生物医学模型的可能性?
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HBB-deficient Macaca fascicularis monkey presents with human β-thalassemia.HBB基因缺陷的食蟹猴表现出人类β地中海贫血症状。
Protein Cell. 2019 Jul;10(7):538-542. doi: 10.1007/s13238-019-0627-y.
2
Identification of preexisting adaptive immunity to Cas9 proteins in humans.鉴定人类对 Cas9 蛋白的预先存在的适应性免疫。
Nat Med. 2019 Feb;25(2):249-254. doi: 10.1038/s41591-018-0326-x. Epub 2019 Jan 28.
3
NP220 mediates silencing of unintegrated retroviral DNA.NP220 介导未整合逆转录病毒 DNA 的沉默。
Nature. 2018 Dec;564(7735):278-282. doi: 10.1038/s41586-018-0750-6. Epub 2018 Nov 28.
4
Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing.Cre 依赖性 Cas9 表达猪可实现高效的体内基因组编辑。
Genome Res. 2017 Dec;27(12):2061-2071. doi: 10.1101/gr.222521.117. Epub 2017 Nov 16.
5
Revealing the complexity of retroviral repression.揭示逆转录病毒抑制的复杂性。
Cell. 2015 Sep 24;163(1):30-2. doi: 10.1016/j.cell.2015.09.014.
6
Histone H3.3 is required for endogenous retroviral element silencing in embryonic stem cells.组蛋白H3.3是胚胎干细胞中内源性逆转录病毒元件沉默所必需的。
Nature. 2015 Jun 11;522(7555):240-244. doi: 10.1038/nature14345. Epub 2015 May 4.
7
Transgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vector.用基于猴免疫缺陷病毒的载体将基因转移到早期胚胎中,从而产生转基因恒河猴。
Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17663-7. doi: 10.1073/pnas.1006563107. Epub 2010 Sep 24.
8
Silencing and variegation of gammaretrovirus and lentivirus vectors.γ逆转录病毒载体和慢病毒载体的沉默与斑驳现象。
Hum Gene Ther. 2005 Nov;16(11):1241-6. doi: 10.1089/hum.2005.16.1241.
9
The degree of phenotypic correction of murine beta -thalassemia intermedia following lentiviral-mediated transfer of a human gamma-globin gene is influenced by chromosomal position effects and vector copy number.慢病毒介导的人γ-珠蛋白基因转移后,中间型小鼠β地中海贫血的表型校正程度受染色体位置效应和载体拷贝数的影响。
Blood. 2003 Mar 15;101(6):2175-83. doi: 10.1182/blood-2002-07-2211. Epub 2002 Oct 31.
10
Retrovirus vector silencing is de novo methylase independent and marked by a repressive histone code.逆转录病毒载体沉默不依赖于从头甲基化酶,并以抑制性组蛋白密码为特征。
EMBO J. 2000 Nov 1;19(21):5884-94. doi: 10.1093/emboj/19.21.5884.