Wong Kin-Weng, Li Yi-Ju, Yang Hui-Ci, Chien Chi-Sheng, Kao Li-Ting, Lin Ting-Sheng, Yang Tsung-Ying, Shih Chi-Jen
Department of Orthopedics, Chi Mei Medical Center, Tainan, Taiwan.
Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
J Dent Sci. 2025 Jan;20(1):510-521. doi: 10.1016/j.jds.2024.04.029. Epub 2024 May 8.
BACKGROUND/PURPOSE: Various pulp-covering materials offer advantages in regenerative root canal treatment, but each has limitations, highlighting the need for more effective antibacterial strategies for pulp repair and regeneration. Mesoporous bioactive glasses (MBG) show significant biological activity, making them valuable in tissue/dental repair. Silver-incorporated MBG exhibits promising antibacterial effects against various bacteria; copper ions are crucial in regulating angiogenesis signals. Co-loading copper and silver in bioactive glasses has been explored to address clinical challenges. This study modified the preparation of silver-copper bimetallic mesoporous bioactive glass, analyzing their textural properties and antibacterial activity against .
The silver-copper co-loaded bioactive glass (designated as AgCu/80S) was synthesized using a sol-gel technique with modifications. Textural analyses were carried out via X-ray diffraction, UV-Vis spectroscopy, Brunauer-Emmett-Teller analysis, and transmission electron microscope. The ion-releasing activity determined using inductively coupled plasma-mass spectrometry, and the antibacterial activity against was assessed through disk diffusion and kinetic bacterial growth curve.
The modification led to weaker crystallization of calcium silicate, altering ion-releasing and antibacterial activities. Ag3Cu2/80S exhibited the highest released silver ion concentration at 112.6 ppm, with an inhibition zone of 9.09 ± 0.09 mm in disk diffusion assays. However, the inhibition zone of Ag2Cu3/80S was 9.92 ± 0.04 mm, implying that the antibacterial activity may not only be influenced by silver ions.
The AgCu/80S showed a potential antibacterial activity against , whereas further research on AgCu/80S glasses is necessary to optimize ion release conditions, assess bioactivities, and explore potential dental applications.
背景/目的：各种牙髓覆盖材料在牙髓再生治疗中具有优势，但每种材料都有局限性，这凸显了需要更有效的抗菌策略来促进牙髓修复和再生。介孔生物活性玻璃（MBG）具有显著的生物活性，使其在组织/牙齿修复中具有重要价值。含银介孔生物活性玻璃对多种细菌具有良好的抗菌效果；铜离子在调节血管生成信号方面至关重要。为应对临床挑战，人们已探索在生物活性玻璃中共负载铜和银。本研究对银铜双金属介孔生物活性玻璃的制备进行了改进，并分析了其结构性质和对[具体细菌名称未给出]的抗菌活性。
采用溶胶 - 凝胶技术并进行改进，合成了银铜共负载生物活性玻璃（命名为AgCu/80S）。通过X射线衍射、紫外 - 可见光谱、布鲁诺尔 - 埃米特 - 泰勒分析和透射电子显微镜进行结构分析。使用电感耦合等离子体质谱法测定离子释放活性，并通过纸片扩散法和细菌生长动力学曲线评估对[具体细菌名称未给出]的抗菌活性。
该改进导致硅酸钙结晶减弱，改变了离子释放和抗菌活性。Ag3Cu2/80S在纸片扩散试验中释放的银离子浓度最高，为112.6 ppm，抑菌圈为9.09±0.09 mm。然而，Ag2Cu3/80S的抑菌圈为9.92±0.04 mm，这意味着抗菌活性可能不仅受银离子影响。
AgCu/80S对[具体细菌名称未给出]显示出潜在的抗菌活性，而有必要对AgCu/80S玻璃进行进一步研究，以优化离子释放条件、评估生物活性并探索潜在的牙科应用。
