Yuan Yiwei, Zhang Yu, Hu Chunli, Wang Zhenyu, Xu Qianqian, Pan Ruili, Li Xiaojun, Christian Mark, Zhu Peinan, Zhao Jin
Institute of Food Nutrition and Quality Safety, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, 310018, China.
Food and Drug Inspection and Testing Center, Chunan, Zhejiang, 311700, China.
Food Funct. 2025 Feb 17;16(4):1347-1359. doi: 10.1039/d4fo04889j.
An effective intervention for obesity without side effects is needed. Chrysanthemum may be the preferred choice due to its influence in the improvement of glycolipid metabolism. This study assessed the efficacy of chrysanthemum and its flavonoids in mitigating high-fat diet (HFD) induced obesity, focusing on the integrity of the intestinal barrier, inflammation, and gut microbiota. Fifty male C57BL/6J mice were divided into 5 groups randomly: normal control (NC), HFD, HFD with chrysanthemum aqueous extract (CM), HFD with a low-dose flavonoid extract of chrysanthemum (FLL), and HFD with a high-dose flavonoid extract of chrysanthemum (FLH). The results showed that after 9 weeks of intervention with CM, FLL and FLH, the body weight and blood lipid levels of mice were reduced. The chrysanthemum treatment regimens down-regulated the gene expression and protein levels of TLR4, MyD88, TRAF6 and NF-κB, upregulated the gene expression levels of ZO-1 and occludin, and decreased the levels of LPS and diamine oxidase (DAO) in the serum. With CM, FLL and FLH, the levels of the inflammatory factors IL-1β, TNF-α, and IL-6 were decreased, and the abundance of pernicious bacteria , and was decreased. Notably, the purified chrysanthemum flavonoid extract showed greater effects as compared to the CM. The study demonstrated that chrysanthemum extracts could achieve anti-obesity effects by strengthening the intestinal barrier function, relieving inflammation and modulating the gut microbial composition.
需要一种无副作用的有效肥胖干预措施。菊花因其在改善糖脂代谢方面的作用可能是首选。本研究评估了菊花及其黄酮类化合物对减轻高脂饮食（HFD）诱导的肥胖的功效，重点关注肠道屏障的完整性、炎症和肠道微生物群。将50只雄性C57BL/6J小鼠随机分为5组：正常对照组（NC）、高脂饮食组（HFD）、高脂饮食+菊花水提取物组（CM）、高脂饮食+低剂量菊花黄酮提取物组（FLL）和高脂饮食+高剂量菊花黄酮提取物组（FLH）。结果显示，CM、FLL和FLH干预9周后，小鼠体重和血脂水平降低。菊花治疗方案下调了TLR4、MyD88、TRAF6和NF-κB的基因表达和蛋白水平，上调了ZO-1和闭合蛋白的基因表达水平，并降低了血清中LPS和二胺氧化酶（DAO）的水平。使用CM、FLL和FLH后，炎症因子IL-1β、TNF-α和IL-6水平降低，有害菌、和的丰度降低。值得注意的是，纯化的菊花黄酮提取物比CM显示出更大的效果。该研究表明，菊花提取物可通过增强肠道屏障功能、减轻炎症和调节肠道微生物组成来实现抗肥胖作用。
