Neptunitalea lumnitzerae sp. nov. isolated from the phyllosphere of the mangrove Lumnitzera racemosa Willd.

A Gram-stain-negative, rod-shaped, non-motile, aerobic, light-yellow-pigmented bacterium, designated as strain Y10, was isolated from Lumnitzera racemosa leaf in Iriomote island mangrove forests in Japan. The 16S rRNA gene sequence analysis revealed that the isolate Y10 was affiliated with the family Flavobacteriaceae, and the sequence showed the highest sequence identity to that of Neptunitalea chrysea NBRC 110019 (97.2%) and others with below 96% sequence identity. The draft genome analysis revealed that its genomic size is 3.85 Mbp with G + C content 34.1%. The average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between strain Y10 and Neptunitalea chrysea NBRC 110019 was 79.07%, 76.35% and 19.6%, respectively, which were below the thresholds for prokaryotic species delineation (95-96%, 85%, and 70%, respectively). MK-6 was the only menaquinone, and the major cellular fatty acids (> 10% of the total fatty acids) were iso-C, iso-C 3-OH, and summed feature 3 (comprised of Cω7c-iso and C 2-OH). Based on its distinct phylogenetic position and genotypic and phenotypic characteristics, the strain represents a novel species of the genus Neptunitalea, for which the name Neptunitalea lumnitzerae sp. nov. is proposed. The type strain is Y10 (= DSM 110280 = NBRC 115783).