Lopez Pierre, Fradet Benjamin, Coffion Lucie, Bernardet Jean-François, Saulnier Denis, Duchaud Eric
Ifremer, IRD, Institut LouisMalard, Universit Polynsie franaise, EIO, F98719 Taravao, Tahiti, Polynsie Franaise, France.
Universit Paris-Saclay, INRAE, UVSQ, VIM, Jouy-en-Josas, France.
Int J Syst Evol Microbiol. 2025 Jan;75(1). doi: 10.1099/ijsem.0.006605.
Ten novel Gram-negative, aerobic, non-sporulating, yellow-pigmented rod-shaped bacterial strains motile by gliding were isolated from marine organisms/environments in French Polynesia. Three of them designated as 190524A05c, 190524A02b and 190130A14a were retrieved from orbicular batfish () mucus. Online database comparisons using 16S rRNA amplicons resulted in over 95% similarity to the genus . Phylogenetic analyses based on 679 concatenated core protein sequences revealed that strains 190524A05c, 190524A02b and 190130A14a showed the highest similarity to DSM 14836, LMG 27422 and S2-2, respectively. Digital DNA-DNA hybridization and average nt identity values between strains 190524A05c, 190524A02b and 190130A14a and other type strains were less than 76.25 and 24.1%, respectively. The DNA G+C content was 31.48, 30.66 and 31.98 mol% for strains 190524A05c, 190524A02b and 190130A14a, respectively. Menaquinone-6 was detected as the major isoprenoid quinone in these three strains. The major polar lipids (phosphatidylethanolamine and aminophospholipid) were similar to the chemotaxonomic profile of other species of the genus . Strain 190524A05c contained summed feature 3 (comprising C ω7c and/or iso-C 2-OH), iso-C G, iso-C and iso-C 3-OH as the major cellular fatty acids. Strain 190524A02b contained summed feature 3 (comprising C ω7c and/or iso-C 2-OH), iso-C, iso-C G and iso-C 3-OH as the major cellular fatty acids. Strain 190130A14a contained iso-C G, summed feature 3 (comprising C ω7c and/or iso-C 2-OH), iso-C and iso-C 3-OH as the major cellular fatty acids. Based on the phenotypic and molecular features, these three strains represent novel species of the genus for which the names sp. nov., with 190524A05c (= CIP 112470 = DSM 118113) as the type strain; sp. nov., with 190524A02b (= CIP 112469 = DSM 118112) as the type strain; and sp. nov., with 190130A14a (= CIP 112468 = DSM 118111) as the type strain, are proposed.
从法属波利尼西亚的海洋生物/环境中分离出10株新型革兰氏阴性、需氧、无芽孢、产黄色色素的滑行运动杆状细菌菌株。其中3株分别命名为190524A05c、190524A02b和190130A14a，是从圆眼燕魟（）黏液中分离得到的。使用16S rRNA扩增子进行在线数据库比对，结果显示与属的相似度超过95%。基于679个串联核心蛋白序列的系统发育分析表明，菌株190524A05c、190524A02b和190130A14a分别与DSM 14836、LMG 27422和S2-2显示出最高的相似度。190524A05c、190524A02b和190130A14a菌株与其他模式菌株之间的数字DNA-DNA杂交和平均核苷酸同一性值分别低于76.25%和24.1%。190524A05c、190524A02b和190130A14a菌株的DNA G+C含量分别为31.48、30.66和31.98 mol%。在这三株菌株中检测到甲基萘醌-6为主要类异戊二烯醌。主要极性脂质（磷脂酰乙醇胺和氨基磷脂）与属其他物种的化学分类特征相似。菌株190524A05c含有特征性总和3（包含C ω7c和/或异-C 2-OH）、异-C G、异-C和异-C 3-OH作为主要细胞脂肪酸。菌株190524A02b含有特征性总和3（包含C ω7c和/或异-C 2-OH）、异-C、异-C G和异-C 3-OH作为主要细胞脂肪酸。菌株190130A14a含有异-C G、特征性总和3（包含C ω7c和/或异-C 2-OH）、异-C和异-C 3-OH作为主要细胞脂肪酸。基于表型和分子特征，这三株菌株代表属的新物种，为此提出三个新物种名，分别为 sp. nov.，以190524A05c（=CIP 112470 =DSM 118113）作为模式菌株； sp. nov.，以190524A02b（=CIP 112469 =DSM 118112）作为模式菌株；以及 sp. nov.，以190130A14a（=CIP 112468 =DSM 118111）作为模式菌株。
