Ali Lubna Sami, Janabi Ali M
DEPARTMENT OF PHARMACOGNOSY, FACULTY OF PHARMACY, UNIVERSITY OF KUFA, KUFA, IRAQ.
DEPARTMENT OF PHARMACOLOGY AND TOXICOLOGY, FACULTY OF PHARMACY, UNIVERSITY OF KUFA, KUFA, IRAQ.
Wiad Lek. 2024;77(12):2442-2450. doi: 10.36740/WLek/196450.
Aim: Testing Cordia myxa extract on colon cancer cell line and caspase-3 gene and COX-2 protein expression.
Materials and Methods: This study used Cordia myxa ethanolic extract at various dosages on SW480 cells. Cell proliferation was measured using MTT, also examined effect of Cordia myxa extract on caspase-3 gene expression using quantitative real-time polymerase chain reaction. The Elisa technique measured cox2 protein.
Results: Cell proliferation tests showed the dose-dependent anticancer effects of Cordia myxa ethanolic extract. Cordia myxa extract also upregulates caspase-3 gene expression and decreases COX-2 protein levels.
Conclusions: Cordia myxa may be a good choice for colon cancer research and anticancer drug development. This plant may cure cancer because of its antioxidant, anti-inflammatory, and anticancer properties.
测试绒毛破布木提取物对结肠癌细胞系以及半胱天冬酶 - 3基因和环氧化酶 - 2蛋白表达的影响。
材料与方法：本研究使用不同剂量的绒毛破布木乙醇提取物作用于SW480细胞。采用MTT法检测细胞增殖情况，同时运用定量实时聚合酶链反应检测绒毛破布木提取物对半胱天冬酶 - 3基因表达的影响。采用酶联免疫吸附测定技术检测环氧化酶 - 2蛋白。
结果：细胞增殖试验显示绒毛破布木乙醇提取物具有剂量依赖性抗癌作用。绒毛破布木提取物还上调半胱天冬酶 - 3基因表达并降低环氧化酶 - 2蛋白水平。
结论：绒毛破布木可能是结肠癌研究和抗癌药物开发的良好选择。这种植物因其抗氧化、抗炎和抗癌特性可能具有治疗癌症的作用。
