He Yanfei, Zhang Dongdong, Wu Qiong, Du Gengan, Liu Ruoting, Zhou Xianqing, Zhang Yurong
Engineering Research Center of Grain Storage and Security of Ministry of Education, Henan Provincial Engineering Technology Research Center on Grain Post Harvest, School of Food and Strategic Reserves, Henan University of Technology, Lianhua Road 100, Zhengzhou High-Tech Development Zone, Zhengzhou, 450001, Henan, China.
Engineering Research Center of Grain Storage and Security of Ministry of Education, Henan Provincial Engineering Technology Research Center on Grain Post Harvest, School of Food and Strategic Reserves, Henan University of Technology, Lianhua Road 100, Zhengzhou High-Tech Development Zone, Zhengzhou, 450001, Henan, China.
Talanta. 2025 May 15;287:127620. doi: 10.1016/j.talanta.2025.127620. Epub 2025 Jan 20.
Aflatoxin B1 (AFB1) has strong carcinogenicity, mutagenicity, and teratogenicity even at low concentrations, presenting a major risk to food safety and human health, hence, it is crucial to develop a sensitive detection technique for AFB1. Consequently, cadmium telluride (CdTe) quantum dots conjugated with AFB1 aptamers serve as fluorescent signal probes, whereas FeO@UiO-66-NH nanocomplexes are employed as magnetic carriers and fluorescence quenchers. FeO@UiO-66-NH reduces background signal interference, thereby enhancing detection sensitivity and Förster Resonance Energy Transfer (FRET) efficiency. We have developed a highly sensitive and selective magnetron fluorescence aptasensor based on FRET for the specific detection of AFB1. Under the ideal experimental conditions, the linear range of AFB1 is 0.001-200 ng/mL, with a linear equation of y = 0.16x+0.65 (R = 0.996), and the detection limit is 0.012 ng/mL. The aptasensor demonstrated superior selectivity, reproducibility, and stability for AFB1, the recoveries for real samples of corn and wheat ranged from 95 % to 103 %, demonstrating the feasibility and practicality of the sensor. Furthermore, the accuracy of the aptasensor was verified by comparison with the results of high performance liquid chromatography, indicating that the aptasensor holds significant potential for practical detection applications.
黄曲霉毒素B1（AFB1）即使在低浓度下也具有很强的致癌性、致突变性和致畸性，对食品安全和人类健康构成重大风险，因此，开发一种灵敏的AFB1检测技术至关重要。因此，与AFB1适配体共轭的碲化镉（CdTe）量子点用作荧光信号探针，而FeO@UiO-66-NH纳米复合物用作磁性载体和荧光猝灭剂。FeO@UiO-66-NH减少了背景信号干扰，从而提高了检测灵敏度和荧光共振能量转移（FRET）效率。我们基于FRET开发了一种用于特异性检测AFB1的高灵敏度和选择性的磁控荧光适配体传感器。在理想实验条件下，AFB1的线性范围为0.001-200 ng/mL，线性方程为y = 0.16x+0.65（R = 0.996），检测限为0.012 ng/mL。该适配体传感器对AFB1表现出优异的选择性、重现性和稳定性，玉米和小麦实际样品的回收率在95%至103%之间，证明了该传感器的可行性和实用性。此外，通过与高效液相色谱结果比较验证了该适配体传感器的准确性，表明该适配体传感器在实际检测应用中具有巨大潜力。
