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使用扫描电子显微镜评估根管治疗期间玷污层去除效率的体外研究。

Evaluation of the efficiency of smear layer removal during endodontic treatment using scanning electron microscopy: an in vitro study.

作者信息

Razumova Svetlana, Brago Anzhela, Kryuchkova Anzhelika, Troitskiy Vasiliy, Bragunova Ruzanna, Barakat Haydar

机构信息

Department of Propedeutics of Dental Diseases, Medical Institute, Peoples' Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya Street, Moscow, 117198, Russian Federation.

Department of Infectious Diseases, Institute of Clinical Medicine, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation.

出版信息

BMC Oral Health. 2025 Jan 28;25(1):151. doi: 10.1186/s12903-025-05510-8.

Abstract

BACKGROUND

The smear layer formed during root canal instrumentation negatively affects root canal irrigation activity, which in turn can affect the treatment prognosis of endodontic treatment.

AIM

The aim of this study is to compare the efficiency of smear layer and debris removal in root canals using different irrigation protocols using scanning electron microscopy (SEM).

MATERIALS AND METHODS

The quality of smear layer removal throughout the root canal was assessed in 30 intact extracted teeth divided into 3 groups according to the irrigation protocol: Group 1: 3% sodium hypochlorite (NaOCL) alternately with 17% ethylenediaminetetraacetic acetate (EDTA) was used. Group 2: 3% NaOCL alternately with 9% etidronate solution diluted with distilled water and Group 3: 9% etidronate solution diluted in 3% NaOCL. The quality of smear layer removal was assessed using scanning electron microscopy (SEM). The nonparametric Mann-Whitney test was used to compare the protocols of irrigations.

RESULTS

The study showed significant differences in the quality of smear layer removal in the apical part of the root canal. The best results were obtained in the groups using 9% etidronate and 9% etidronate solution diluted in 3% sodium hypochlorite. Conducting a comparative evaluation of the effectiveness of removing the smear layer using 3% NaOCL + 17% EDTA, 3% NaOCL + 9% etidronic acid diluted with distilled water, 3% NaOCL + 9% etidronic acid diluted with 3% hypochlorite showed greater effectiveness of protocols using etidronate. When comparing statistical data, protocols using etidronate were 4 times more effective than EDTA.

CONCLUSION

The smear layer formed during mechanical treatment of the root canal was better removed during irrigation with etidronate solution.

摘要

背景

根管预备过程中形成的玷污层会对根管冲洗效果产生负面影响,进而可能影响牙髓治疗的预后。

目的

本研究旨在通过扫描电子显微镜(SEM)比较不同冲洗方案去除根管内玷污层和碎屑的效率。

材料与方法

选取30颗完整拔除的牙齿,根据冲洗方案分为3组,评估根管内玷污层的去除质量。第1组:交替使用3%次氯酸钠(NaOCl)和17%乙二胺四乙酸(EDTA)。第2组:交替使用3% NaOCl和用蒸馏水稀释的9%依替膦酸溶液。第3组:在3% NaOCl中稀释9%依替膦酸溶液。使用扫描电子显微镜(SEM)评估玷污层的去除质量。采用非参数曼-惠特尼检验比较冲洗方案。

结果

研究表明,根管根尖部玷污层的去除质量存在显著差异。使用9%依替膦酸和在3%次氯酸钠中稀释的9%依替膦酸溶液的组效果最佳。对使用3% NaOCl + 17% EDTA、3% NaOCl + 用蒸馏水稀释的9%依替膦酸、3% NaOCl + 用3%次氯酸盐稀释的9%依替膦酸去除玷污层的效果进行比较评估,结果显示使用依替膦酸的方案效果更佳。比较统计数据时,使用依替膦酸的方案比EDTA有效4倍。

结论

在根管机械治疗过程中形成的玷污层,在用依替膦酸溶液冲洗时能更好地被去除。

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