NLRP3炎性小体及相关细胞分子在老年人群早期糖尿病肾病中的临床意义
Clinical significance of NLRP3 inflammasome and related cell molecules in early diabetic kidney disease in elderly population.
作者信息
Li Xiaoli, Liu Shiwei, Huangfu Jinrong, Lai Nannan, Shang Yan
机构信息
Third Hospital of Shanxi Medical University, Shanxi Academy of Medical Sciences, Shanxi Bethune Hospital, Tongji Shanxi Hospital, Department of Endocrinology, Taiyuan, China.
Third Hospital of Shanxi Medical University, Shanxi Academy of Medical Sciences, Shanxi Bethune Hospital, Tongji Shanxi Hospital, Department of Nephrology, Taiyuan, China.
出版信息
J Med Biochem. 2024 Nov 16;43(6):828-834. doi: 10.5937/jomb0-45950.
BACKGROUND
The paper aims to investigate the expression level of NLRP3 inflammasome and its related cell molecules in early diabetes kidney disease (EDKD) in the elderly and its clinical application value.
METHODS
From October 2021 to April 2023, 50 elderly patients with T2DM (T2DM group), 50 elderly patients with EDKD (EDKD group) and 50 elderly people who passed the health check-up (healthy group) were chosen as the study subjects. Plasma NLRP3 inflammasome and related cells (blood leukocyte count, monocyte count, lymphocyte count) molecular (NT-proBNP and others) levels are tested, and Pearson correlation analysis is utilized to explore the correlation among plasma NLRP3 inflammasome and related cells, molecules, and renal function indicators (UACR, BUN, Ucr) in elderly patients with EDKD.
RESULTS
(1) The three groups' comparison in HbA1c, FIns, HOMA-IR, UACR, BUN, Ucr, SOD, MCP-1, and TNF-a levels were with P<0.05. The levels of TG and LDL-C in the EDKD group were higher than those in the T2DM and the healthy groups; the levels of FPG, HbA1c, FINs, HOMA-IR, UACR, SOD, MCP-1, TNF-a in the EDKD and T2DM groups were higher than those in the healthy group, while SOD was smaller than that in the healthy group; the levels of BUN, Ucr, hs-CRP, FPG, HbA1c, FINs, HOMA-IR, UACR, SOD, MCP-1, TNF-a in the EDKD group were higher than those in the T2DM group, while SOD was smaller than that in the T2DM group. The above results were with P<0.05. (2) It has P<0.05 in Monocyte count, NLRP3, NT-proBNP, caspase-1, ASC and others in the three groups. Those in the EDKD and T2DM groups were higher than those in the healthy group. The levels of these indicators in the EDKD group were higher than those in the T2DM group, with P<0.05. NLRP3, Caspase-1, ASC, IL1b, and IL-18 were positively correlated with UACR, BUN, and Ucr in the EDKD group. All the above differences were P<0.05.
CONCLUSIONS
NLRP3 inflammasome and its related molecules caspase-1, ASC, IL-1b, IL-18 and other levels increase in early elderly EDKD and are closely related to the severity of EDKD.
背景
本文旨在探讨老年早期糖尿病肾病（EDKD）中NLRP3炎性小体及其相关细胞分子的表达水平及其临床应用价值。
方法
选取2021年10月至2023年4月的50例老年2型糖尿病患者（T2DM组）、50例老年EDKD患者（EDKD组）和50例健康体检老人（健康组）作为研究对象。检测血浆NLRP3炎性小体及相关细胞（血白细胞计数、单核细胞计数、淋巴细胞计数）分子（NT-proBNP等）水平，采用Pearson相关分析探讨老年EDKD患者血浆NLRP3炎性小体及相关细胞、分子与肾功能指标（UACR、BUN、Ucr）之间的相关性。
结果
（1）三组在HbA1c、FIns、HOMA-IR、UACR、BUN、Ucr、SOD、MCP-1和TNF-a水平上比较，P<0.05。EDKD组TG和LDL-C水平高于T2DM组和健康组；EDKD组和T2DM组FPG、HbA1c、FINs、HOMA-IR、UACR、SOD、MCP-1、TNF-a水平高于健康组，而SOD低于健康组；EDKD组BUN、Ucr、hs-CRP、FPG、HbA1c、FINs、HOMA-IR、UACR、SOD、MCP-1、TNF-a水平高于T2DM组，而SOD低于T2DM组。上述结果P<0.05。（2）三组单核细胞计数、NLRP3、NT-proBNP、caspase-1、ASC等比较，P<0.05。EDKD组和T2DM组高于健康组。EDKD组这些指标水平高于T2DM组，P<0.05。EDKD组NLRP3、Caspase-1、ASC、IL1b和IL-18与UACR、BUN和Ucr呈正相关。上述差异均P<0.05。
结论
老年EDKD早期NLRP3炎性小体及其相关分子caspase-1、ASC、IL-1b、IL-18等水平升高，且与EDKD严重程度密切相关。
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