Budu Martison, Boakye Patrick, Bentil Joseph A
Department of Biochemistry and Biotechnology, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Department of Chemical Engineering, College of Engineering, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
ScientificWorldJournal. 2025 Jan 21;2025:2296078. doi: 10.1155/tswj/2296078. eCollection 2025.
Bioethanol production is one of the key alternatives for fossil fuel use due to climate change. The study seeks to upscale tailor-made onsite enzyme blends for the bioconversion of cassava peels to bioethanol in simultaneous saccharification and fermentation (SSF) process using cassava peels-degrading fungi. The starch and cellulose contents of peels were determined. The results showed significant levels of cellulose (39.78%) and starch (31.21%), indicating that cassava peels are valuable raw materials for bioethanol production. To investigate cassava-degrading microbes, demonstrated the highest enzyme activity with a diameter of zone of clearance of 16 mm. Tailor-made enzyme blends were produced with the . using various substrate concentrations (1%, 3%, 5%, 8%, and 10%) of milled cassava peels at periods of 2, 4, 6, 8, and 10 days with a spore concentration of 2.1 × 10 cells/mL. The amylolytic and cellulolytic activities were determined as 4.759 U/mL and 4.265 U/mL, respectively, at 5% and 6-day optimal conditions. The enzyme blend was upscaled using three fermentation vessels, thus 0.250 L flask, 1.0 L flask, and 10 L fermenter at optimized conditions in the SSF process for bioethanol production. These optimal conditions were firstly applied to a 0.250 L flask in the SSF process, a fixed enzyme dose of 20 mL and 1.5 g of with varying substrate concentrations of 5%, 10%, and 20% and ethanol analyzed daily for 10 days. The theoretical ethanol yields recorded were 15.64%, 16.02%, and 14.91% for 0.250, 1, and 10 volumes obtained at different fermentation days. These optimized conditions demonstrate the potential for industrial bioethanol production in Ghana.
由于气候变化，生物乙醇生产是化石燃料使用的关键替代方案之一。该研究旨在扩大定制的现场酶混合物的规模，以便在使用木薯皮降解真菌的同步糖化和发酵（SSF）过程中，将木薯皮生物转化为生物乙醇。测定了木薯皮的淀粉和纤维素含量。结果显示纤维素（39.78%）和淀粉（31.21%）含量显著，表明木薯皮是生物乙醇生产的宝贵原料。为了研究木薯降解微生物，其显示出最高的酶活性，透明圈直径为16毫米。使用不同底物浓度（1%、3%、5%、8%和10%）的研磨木薯皮，在2、4、6、8和10天的时间段内，孢子浓度为2.1×10细胞/毫升，制备定制的酶混合物。在5%和6天的最佳条件下，淀粉分解活性和纤维素分解活性分别测定为4.759 U/mL和4.265 U/mL。在SSF生物乙醇生产过程的优化条件下，使用三个发酵容器扩大酶混合物的规模，即0.250升烧瓶、1.0升烧瓶和10升发酵罐。这些最佳条件首先应用于SSF过程中的0.250升烧瓶，固定酶剂量为20毫升，1.5克木薯皮，底物浓度分别为5%、10%和20%，并在10天内每天分析乙醇含量。在不同发酵天数获得的0.250、1和10体积的理论乙醇产量分别为15.64%、16.02%和14.91%。这些优化条件证明了加纳工业生物乙醇生产的潜力。
