Amalia Nadya Rafika, Budhy Theresia Indah, Ridwan Rini Devijanti, Rianti Devi, Bramantoro Taufan, Luthfi Muhammad, Ramadhani Nastiti Faradilla, Pramusita Adya, Putranti Nurul Aisyah Rizki, Nugraha Albertus Putera, Situmorang Putri Cahaya, Shariff Khairul Anuar, Nugraha Alexander Patera
Dental Health Science Study Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Oral and Maxillofacial Pathology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
J Oral Biol Craniofac Res. 2025 May-Jun;15(3):576-584. doi: 10.1016/j.jobcr.2025.03.013. Epub 2025 Mar 28.
Propolis from Sulawesi's stingless bees () contains antioxidants, more flavonoids than propolis from Apis bees, and the antibacterial ability.
to examine the antibacterial properties of Propolis Nanoemulsion Extract (PNE), which is extracted from the Celebes Stingless Bee (), in relation to the periodontopathogen bacteria such as (Fn), (Pg), (Aa), and (Pi). This investigation also examines PNE's phytochemistry, particle size analysis (PSA), and zeta potential.
The maceration process with 96 % ethanol was used to create PNE from Celebes stingless bee (), which was then subjected to zeta potential measurement and PSA. Phytochemical analysis was used to identify phytochemical constituents in the PNE (T. biroi). Diffusion zone, minimum bactericidal concentration (MBC), and minimum inhibitory concentration (MIC) were used to assess antibacterial efficacy against Aa, Pg, Pi, and Fn. Furthermore, the statistical analysis was used to extract the data.
Phenols, alkaloids, and flavonoids were identified; however, triterpenoids and saponins were not. Between 151.28 and 182.2 diameter nanometers (d.nm) was the range of the PNE's diameter. At 1.56 % propolis () concentration, the MIC, MBC, and diffusion zone analysis performed better than at 0.76 %, with a significant difference (p:0.01; p < 0.05) to Aa, Pg, Pi, and Fn.
The highest antibacterial activity against Aa, Pg, Pi, and Fn as periodontopathogen bacteria is demonstrated by alkaloids, flavonoids, and phenols in PNE from Celebes () at a concentration of 1.56 %.
来自苏拉威西无刺蜂的蜂胶含有抗氧化剂，黄酮类化合物比蜜蜂蜂胶更多，且具有抗菌能力。
研究从西里伯斯无刺蜂中提取的蜂胶纳米乳提取物（PNE）对牙周病原菌如具核梭杆菌（Fn）、牙龈卟啉单胞菌（Pg）、伴放线聚集杆菌（Aa）和中间普氏菌（Pi）的抗菌特性。本研究还检测了PNE的植物化学性质、粒径分析（PSA）和zeta电位。
采用96%乙醇浸渍法从西里伯斯无刺蜂制备PNE，然后进行zeta电位测量和PSA。采用植物化学分析鉴定PNE（T. biroi）中的植物化学成分。采用扩散区、最低杀菌浓度（MBC）和最低抑菌浓度（MIC）评估对Aa、Pg、Pi和Fn的抗菌效果。此外，采用统计分析提取数据。
鉴定出了酚类、生物碱和黄酮类化合物；但未鉴定出三萜类和皂苷类化合物。PNE的直径范围在151.28至182.2纳米（d.nm）之间。在蜂胶浓度为1.56%时，MIC、MBC和扩散区分析的效果优于0.76%时对Aa、Pg、Pi和Fn的效果，差异有统计学意义（p:0.01；p < 0.05）。
西里伯斯（）PNE中浓度为1.56%的生物碱、黄酮类化合物和酚类化合物对作为牙周病原菌的Aa、Pg、Pi和Fn具有最高的抗菌活性。
