Önen Selin, Gizer Merve, Çolak İmran Özge, Korkusuz Petek
METU MEMS Center, Ankara, Turkey.
Department of Stem Cell Sciences, Graduate School of Health Sciences, Hacettepe University, Ankara, Turkey.
Adv Exp Med Biol. 2025;1479:59-72. doi: 10.1007/5584_2024_844.
Male factor accounts for 30-50% of infertility cases and may occur due to congenital anomalies or acquired disorders. In such infertility cases where a limited number of mature sperm is produced, a solution is offered to patients with ART applications; however, these methods are inadequate in patients with germ cell aplasia due to damaged microenvironment. Since monolayer cell culture and static culture conditions do not provide the physical conditions of the 3D microenvironment, they have a limited effect on ensuring the execution of in vitro spermatogenesis properly. For this reason, current treatment approaches turn to biomaterial-implemented, microfluidic, and bioreactor systems where 3D physical conditions are provided. This book chapter focuses on static and dynamic culture conditions, as well as the use of biomaterials to increase the success of ex vivo spermatogenesis and microfluidic device-assisted sperm selection in ART.
男性因素占不孕病例的30%-50%，可能由先天性异常或后天性疾病引起。在产生成熟精子数量有限的此类不孕病例中，辅助生殖技术（ART）应用为患者提供了一种解决方案；然而，对于因微环境受损导致生殖细胞发育不全的患者，这些方法并不充分。由于单层细胞培养和静态培养条件无法提供三维微环境的物理条件，它们在确保体外精子发生正常进行方面的作用有限。因此，当前的治疗方法转向了提供三维物理条件的生物材料植入、微流体和生物反应器系统。本章重点介绍静态和动态培养条件，以及使用生物材料提高体外精子发生成功率和在辅助生殖技术中利用微流体装置辅助精子筛选的情况。
