Quan Zihan, Fan Songqing, Zheng Hongmei, Ning Yue, Yang Yang
Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Department of Pathology, The Fourth People's Hospital of Longgang District, Shenzhen, Guangdong, China.
Cancer Biol Ther. 2025 Dec;26(1):2458773. doi: 10.1080/15384047.2025.2458773. Epub 2025 Jan 29.
Membrane-associated RING-CH8 (MARCH8) is a member of the recently discovered MARCH family of ubiquitin ligases. MARCH8 has been shown to participate in immune responses. However, the role of MARCH8 in prognosis and immunology in human cancers remains largely unknown. The expression of MARCH8 protein was detected via immunohistochemistry in non-small cell lung cancer (NSCLC) and non-cancerous lung tissues. The study investigated the role of MARCH8 in tumor immunity through pan-cancer analysis of multiple databases. MARCH8 genetic alternations and expression were explored with the cBioPortal, GTEx, and TCGA databases. We investigated the role of MARCH8 expression in clinical relevance, prognosis, tumor immune microenvironment, immune checkpoint (ICP) with a series of bioinformatics tools and methods, such as TISIDB database, ESTIMATE, and CIBERSORT method. MARCH8 expression showed cancer-specific dysregulation and was associated with the prognosis of patients in various cancers. MARCH8 was related to the tumor microenvironment and participated in tumor immune regulation. Furthermore, low expression of MARCH8 was associated with poor prognosis and might serve as an independent prognostic biomarker for NSCLC patients. The comprehensive pan-cancer analysis revealed the potential of MARCH8 in tumor-targeted therapy, and suggested MARCH8 as a promising prognostic and immunological pan-cancer biomarker.
膜相关RING-CH8（MARCH8）是最近发现的泛素连接酶MARCH家族的成员。已证明MARCH8参与免疫反应。然而，MARCH8在人类癌症的预后和免疫学中的作用仍然很大程度上未知。通过免疫组织化学检测非小细胞肺癌（NSCLC）和非癌性肺组织中MARCH8蛋白的表达。该研究通过对多个数据库的泛癌分析来研究MARCH8在肿瘤免疫中的作用。利用cBioPortal、GTEx和TCGA数据库探索MARCH8的基因改变和表达。我们使用一系列生物信息学工具和方法，如TISIDB数据库、ESTIMATE和CIBERSORT方法，研究了MARCH8表达在临床相关性、预后、肿瘤免疫微环境、免疫检查点（ICP）中的作用。MARCH8表达显示出癌症特异性失调，并与各种癌症患者的预后相关。MARCH8与肿瘤微环境相关，并参与肿瘤免疫调节。此外，MARCH8低表达与预后不良相关，可能作为NSCLC患者的独立预后生物标志物。全面的泛癌分析揭示了MARCH8在肿瘤靶向治疗中的潜力，并表明MARCH8是一种有前景的预后和免疫泛癌生物标志物。
