Zhang Chenqiu, Chen Tianjian, Li Zhiyu, Lu Qing, Luo Xiaotong, Cai Sihui, Zhou Jie, Ren Jian, Cui Jun
MOE Key Laboratory of Gene Function and Regulation, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, 510275, China.
State Key Laboratory of Oncology in South China, Cancer Center, Collaborative Innovation Center for Cancer Medicine, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Adv Biotechnol (Singap). 2024 Sep 3;2(3):29. doi: 10.1007/s44307-024-00036-6.
Although significant progress of clinical strategy has been made in gene editing and cell engineering in immunotherapy, it is now apparent that design and modification in terms of complex signaling pathways and motifs on medical synthetic biology are still full of challenges. Innate immunity, the first line of host defense against pathogens, is critical for anti-pathogens immune response as well as regulating durable and protective T cell-mediated anti-tumor responses. Here, we introduce DSCI (Database of Synthetic Biology Components for Innate Immunity, https://dsci.renlab.cn/ ), a web-accessible and integrative database that provides better insights and strategies for innate immune signaling circuit design in biosynthesis. Users can interactively navigate comprehensive and carefully curated components resources that presented as visualized signaling motifs that participate in innate immunity. The current release of DSCI incorporates 1240 independent components and more than 4000 specific entries contextually annotated from public literature with experimental verification. The data integrated into DSCI includes the components of pathways, relationships between regulators, signal motifs based on regulatory cascades, and loop graphs, all of which have been comprehensively annotated to help guide modifications to gene circuits. With the support of DSCI, users can easily obtain guidance of gene circuits construction to make decision of cell engineering based on innate immunity. DSCI not only provides comprehensive and specialized resource on the biological components of innate immune synthesis, but also serves as a useful tool to offer modification or generation strategies for medical synthetic biology.
尽管在免疫疗法的基因编辑和细胞工程方面临床策略已取得显著进展，但现在很明显，在医学合成生物学中，针对复杂信号通路和基序的设计和修饰仍然充满挑战。先天免疫作为宿主抵御病原体的第一道防线，对于抗病原体免疫反应以及调节持久且具有保护性的T细胞介导的抗肿瘤反应至关重要。在此，我们介绍DSCI（先天免疫合成生物学组件数据库，https://dsci.renlab.cn/），这是一个可通过网络访问的综合数据库，它为生物合成中的先天免疫信号回路设计提供了更好的见解和策略。用户可以交互式浏览全面且精心策划的组件资源，这些资源以参与先天免疫的可视化信号基序呈现。DSCI的当前版本纳入了1240个独立组件以及4000多个从经实验验证的公共文献中按上下文注释的特定条目。整合到DSCI中的数据包括信号通路的组件、调节因子之间的关系、基于调节级联的信号基序以及循环图，所有这些都经过了全面注释，以帮助指导基因回路的修饰。在DSCI的支持下，用户可以轻松获得基因回路构建的指导，从而基于先天免疫做出细胞工程决策。DSCI不仅提供了关于先天免疫合成生物成分的全面且专业的资源，还作为一个有用的工具，为医学合成生物学提供修饰或生成策略。
